Role of acid metabolism in Streptomyces coelicolor morphological differentiation and antibiotic biosynthesis

Journal of Bacteriology
P H ViollierC J Thompson

Abstract

Studies of citrate synthase (CitA) were carried out to investigate its role in morphological development and biosynthesis of antibiotics in Streptomyces coelicolor. Purification of CitA, the major vegetative enzyme activity, allowed characterization of its kinetic properties. The apparent K(m) values of CitA for acetyl coenzyme A (acetyl-CoA) (32 microM) and oxaloacetate (17 microM) were similar to those of citrate synthases from other gram-positive bacteria and eukaryotes. CitA was not strongly inhibited by various allosteric feedback inhibitors (NAD(+), NADH, ATP, ADP, isocitrate, or alpha-ketoglutarate). The corresponding gene (citA) was cloned and sequenced, allowing construction of a citA mutant (BZ2). BZ2 was a glutamate auxotroph, indicating that citA encoded the major citrate synthase allowing flow of acetyl-CoA into the tricarboxylic acid (TCA) cycle. Interruption of aerobic TCA cycle-based metabolism resulted in acidification of the medium and defects in morphological differentiation and antibiotic biosynthesis. These developmental defects of the citA mutant were in part due to a glucose-dependent medium acidification that was also exhibited by some other bald mutants. Unlike other acidogenic bald strains, citA and bl...Continue Reading

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Citations

Aug 13, 2008·Applied Microbiology and Biotechnology·Hideaki TakanoKenji Ueda
Jun 27, 2012·Antonie van Leeuwenhoek·Elodie TenconiSébastien Rigali
Dec 4, 2001·Current Opinion in Microbiology·K F Chater
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Jan 2, 2007·Journal of Biotechnology·Alexander KernAnton Glieder
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Mar 16, 2013·FEMS Microbiology Letters·Cyril BontempsPierre Leblond
Nov 27, 2015·Microbiology and Molecular Biology Reviews : MMBR·Essaid Ait BarkaGilles P van Wezel
Oct 30, 2018·Nucleic Acids Research·Klára ŠmídováJiri Vohradsky
Mar 11, 2018·Applied Microbiology and Biotechnology·Howard Ramirez-MaluleRigoberto Ríos-Estepa
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Jun 25, 2018·Archives of Microbiology·Tóshiko Takahashi-ÍñiguezMaría Elena Flores

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