Role of actin-bundling protein Sac6 in growth of Cryptococcus neoformans at low oxygen concentration.

Eukaryotic Cell
Yun C ChangKyung J Kwon-Chung

Abstract

Cryptococcus neoformans, the etiologic agent of cryptococcosis, is an obligately aerobic yeast that inhabits an environmental niche exposed to ambient air. The cell doubling time was significantly prolonged under 1% O(2) relative to that under normoxic conditions. No apparent cell cycle arrest occurred following a shift from ambient air to 1% O(2). However, yeast cells became hypersensitive to the actin monomer-sequestering agent latrunculin A at 1% O(2), indicating that proper actin function is critical for growth at low oxygen concentrations. We showed that Sac6, an actin-binding protein, played an important role in cell growth under low oxygen conditions. Sac6 colocalized with cortical actin patches and with the ring structures between mother cells and buds. Under low oxygen conditions, the sac6 deletion mutant grew poorly, and accumulation of the actin capping protein Cap1 was observed in the vacuole of the sac6Δ strain. Furthermore, endocytic processes were hampered in the sac6Δ mutant, but cell polarity and cytokinesis were not visibly disturbed. The deficiency of endocytosis in the sac6Δ strain could be rescued by 1 M sorbitol under 1% O(2), but growth remained retarded. These results suggest an absence of a direct link ...Continue Reading

References

Mar 1, 1975·Microvascular Research·P NairD Buerk
Dec 1, 1988·The Journal of Cell Biology·D G DrubinD Botstein
Apr 1, 1995·Journal of Clinical Microbiology·F C OddsF Woestenborghs
Mar 1, 1993·Journal of Bacteriology·D L ToffalettiJ R Perfect
Sep 1, 1996·Journal of Medical and Veterinary Mycology : Bi-monthly Publication of the International Society for Human and Animal Mycology·R TanakaK Nishimura
Apr 8, 2000·Biotechnology Progress·T R GervaisJ L Gainer
Mar 15, 2001·Proceedings of the National Academy of Sciences of the United States of America·Y C ChangK J Kwon-Chung
Nov 24, 2001·Respiration Physiology·M Erecińska, I A Silver
Jan 15, 2002·Nucleic Acids Research·Hidekazu KuwayamaYoshimasa Tanaka
Jul 19, 2003·The Biochemical Journal·Steven J WinderKathryn R Ayscough
Oct 23, 2003·Annual Review of Cell and Developmental Biology·Asa E Y Engqvist-Goldstein, David G Drubin
Mar 20, 2004·Antonie van Leeuwenhoek·Misako OhkusuKanji Takeo
Sep 15, 2004·Trends in Biochemical Sciences·Martin A Wear, John A Cooper
Sep 9, 2006·Microbiology and Molecular Biology Reviews : MMBR·James B Moseley, Bruce L Goode
May 5, 2007·Annual Review of Biophysics and Biomolecular Structure·Thomas D Pollard
Apr 11, 2008·The Journal of Biological Chemistry·Dana M GheorgheKathryn R Ayscough
Jun 10, 2008·Nature Methods·Julia RiedlRoland Wedlich-Soldner
Oct 25, 2011·Trends in Cell Biology·Jasper Weinberg, David G Drubin
Mar 27, 2012·Eukaryotic Cell·Nora GrahlRobert A Cramer

❮ Previous
Next ❯

Related Concepts

Related Feeds

Actin-binding Proteins

Actin-binding proteins are a component of the actin cytoskeleton that play essential roles in cellular functions such as regulation of actin polymerization, maintenance of cell polarity, gene expression regulation, cell motility and many more functions. Discover the latest research on actin-binding proteins here.