PMID: 9525606Apr 3, 1998Paper

Role of actin microfilaments in Black Creek Canal virus morphogenesis

Journal of Virology
E V RavkovR W Compans

Abstract

We have investigated the involvement of cytoskeletal proteins in the morphogenesis of Black Creek Canal virus (BCCV), a New World hantavirus. Immunofluorescent staining of BCCV-infected cells revealed a filamentous pattern of virus antigen, the appearance of which was sensitive to treatment with cytochalasin D, an actin microfilament-depolymerizing drug. Double immunofluorescence staining of BCCV-infected Vero cells with anti-BCCV nucleocapsid (N) monoclonal antibody and phalloidin revealed a colocalization of the BCCV N protein with actin microfilaments. A similar, though less prominent, filamentous pattern was observed in BHK21 cells transiently expressing the BCCV N protein alone but not in cells expressing the BCCV G1 and G2 glycoproteins. Moreover, the association of the N protein with actin microfilaments was confirmed by coimmunoprecipitation with beta-actin-specific antibody. Treatment of the BCCV-infected Vero cells at 3 days postinfection with cytochalasin D decreased the yield of released BCCV by 94% relative to the yield from untreated cells. Pretreatment of Vero cells with cytochalasin D prior to and during BCCV adsorption and entry had no effect on the outcome of virus production. These results indicate that actin...Continue Reading

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Citations

Jul 18, 2001·The Journal of General Virology·P KaukinenA Plyusnin
Sep 27, 2003·Journal of Virology·Pasi KaukinenAlexander Plyusnin
May 11, 2004·The Journal of Veterinary Medical Science·Hideo KatayamaChieko Kai
Feb 12, 2009·Journal of Biosciences·S ChandyG Sridharan
Aug 9, 2008·The Journal of Biological Chemistry·Yu WangRoberto N De Guzman
Aug 26, 2011·The Journal of General Virology·Cheryl T Walter, John N Barr
Aug 31, 2006·Journal of Virology·Agne AlminaiteAlexander Plyusnin
Apr 9, 2010·Clinical Microbiology Reviews·Colleen B JonssonOlli Vapalahti
Aug 16, 2014·Viruses·Kumiko Yoshimatsu, Jiro Arikawa
Dec 8, 2015·Frontiers in Microbiology·Musalwa MuyangwaAlbert A Rizvanov
Apr 24, 2004·The Journal of General Virology·Sami K J KukkonenAlexander Plyusnin
Sep 11, 2003·Journal of Medical Virology·J J H ChuM L Ng
Nov 23, 2000·Journal of Virology·S F KhaiboullinaS C St Jeor
Feb 13, 2001·Journal of Virology·A AlfadhliE Barklis
Sep 15, 2006·Virus Genes·Lena LindgrenAnna Holmström
Dec 22, 2010·Expert Review of Anti-infective Therapy·Jonas Klingström, Clas Ahlm
May 25, 2012·The Journal of General Virology·Jussi HepojokiAntti Vaheri
Apr 18, 2000·The Journal of General Virology·Q Yao, R W Compans
Feb 1, 2003·The Journal of Biological Chemistry·Rongcun YangRichard B S Roden
Nov 20, 2004·Emerging Infectious Diseases·M L NgE E Ooi
Sep 9, 2010·PLoS Neglected Tropical Diseases·Jia-Li WangJing An
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Aug 29, 2021·Viruses·Kristina MeierMaria Rosenthal
Dec 22, 2005·Virology Journal·Ziying Han, Ronald N Harty

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