Role of ADAMTS-12 in Protecting Against Inflammatory Arthritis in Mice By Interacting With and Inactivating Proinflammatory Connective Tissue Growth Factor

Arthritis & Rheumatology
Jian-Lu WeiChuan-Ju Liu

Abstract

It has been reported that ADAMTS-12 is a susceptibility gene for rheumatoid arthritis (RA) development, and its level is significantly increased in RA patients. In addition, ADAMTS-12 is reported to be required for inflammation in otherwise healthy subjects. This study was undertaken to determine the role of ADAMTS-12 and the underlying mechanisms in the pathogenesis of inflammatory arthritis. The collagen-induced arthritis (CIA) model was established in ADAMTS-12-deficient mice and their control littermates to determine the role of ADAMTS-12 in vivo. Micro-computed tomography scanning was used to demonstrate the destruction of the ankle joint; histologic analysis illustrated synovitis, pannus formation, and bone and cartilage destruction; enzyme-linked immunosorbent assay was performed to measure serum levels of inflammatory cytokines; and protein-protein interaction assays were performed to detect the interactions of ADAMTS-12 and its various deletion mutants with connective tissue growth factor (CTGF). Deficiency of ADAMTS-12 led to accelerated inflammatory arthritis in the CIA mouse model. Loss of ADAMTS-12 caused enhanced osteoclastogenesis. In vitro and in vivo protein-protein interaction assays demonstrated that ADAMTS-1...Continue Reading

References

Jul 3, 2004·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Takashi NishidaMasaharu Takigawa
Apr 1, 2005·Nature·Heather StantonAmanda J Fosang
Apr 6, 2006·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Chuan-Ju LiuPaul E Di Cesare
Apr 14, 2006·The Journal of Biological Chemistry·Chuan-ju LiuPaul E Di Cesare
Jun 5, 2007·Nature Protocols·David D BrandEdward F Rosloniec
Dec 23, 2008·Nature Clinical Practice. Rheumatology·Chuan-Ju Liu
Dec 7, 2010·Biochimie·L KularC Martinerie
Jan 5, 2011·Protein & Cell·Edward A Lin, Chuan-Ju Liu
Jan 17, 2012·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Qiang ZhangYan Wang
Mar 16, 2012·Biomarkers : Biochemical Indicators of Exposure, Response, and Susceptibility to Chemicals·Sittisak HonsawekSaran Tantavisut
Sep 11, 2012·The Journal of Immunology : Official Journal of the American Association of Immunologists·Geneviève PaulissenDidier D Cataldo
Sep 29, 2012·The Journal of Biological Chemistry·Angela Moncada-PazosCarlos López-Otín
Aug 10, 2013·Annals of the Rheumatic Diseases·Yongjie LaiChuan-Ju Liu
Jan 7, 2014·Nature·Yukinori OkadaRobert M Plenge
May 31, 2014·Mediators of Inflammation·Jianlu WeiChuanju Liu
Nov 19, 2014·World Journal of Orthopedics·Kazuhisa NozawaIwao Sekigawa
May 15, 2015·Annals of the Rheumatic Diseases·Josef S SmolenDesirée van der Heijde
May 31, 2015·Genome Biology·Richard KelwickDylan R Edwards
Aug 10, 2016·Annals of the New York Academy of Sciences·Jianlu WeiChuanju Liu
Dec 20, 2016·Annals of the Rheumatic Diseases·Hanna W van SteenbergenAnnette H M van der Helm-van Mil
Feb 19, 2017·Seminars in Immunopathology·Mélissa Noack, Pierre Miossec

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Citations

Nov 5, 2019·Orthopaedic Surgery·Jian-Lu WeiYing-Ze Zhang
Jan 4, 2021·Clinical Rheumatology·Jiaqi RenJinxia Zhao
Feb 6, 2021·Annals of the New York Academy of Sciences·Yuanjing DingChuan-Ju Liu
May 18, 2021·Frontiers in Molecular Biosciences·Yamina MohamediÁlvaro J Obaya
Sep 11, 2021·Frontiers in Cell and Developmental Biology·Kaiwen LiuLei Cheng

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