PMID: 36358Feb 1, 1979

Role of alkaline endonucleases in the release of soluble chromatin from thymus, spleen and liver nuclei of normal and irradiated mice

International Journal of Radiation Biology and Related Studies in Physics, Chemistry, and Medicine
D Suciu

Abstract

Thymus, spleen and liver nuclei released a large fraction of soluble chromatin in vitro when incubation was carried out in sucrose media containing low concentrations of CaCl2 and/or MgCl2. A significant fraction of deoxyribopolynucleotides (DPN) was also extracted from nuclei. After 30 min of incubation at 37 degrees C, the maximum release of soluble chromatin was observed near a pH of 8, which corresponds to the optimum pH of the alkaline endonuclease activity from thymus, spleen and liver. The soluble chromatin and DPN were precipitated by increasing the bivalent ion concentration of the medium. The protein/DNA ratio and the molecular weight of DNA suggest that the soluble chromatin and DPN represent nucleosome-like particles. The release of soluble chromatin in the first 4 hours of incubation was significantly increased if the nuclear fraction was isolated from the thymus and spleen of whole-body irradiated mice (1000 rad). This effect was absent in the liver nuclei.

References

Oct 1, 1978·Nucleic Acids Research·P N BryanD E Olins
Nov 1, 1977·Nucleic Acids Research·H J LiC Chang
Dec 1, 1976·Nucleic Acids Research·A L OlinsD E Olins
Jan 1, 1975·Annual Review of Biochemistry·S C Elgin, H Weintraub
Sep 1, 1975·Nucleic Acids Research·L A Burgoyne, J Mobbs
Apr 1, 1976·Nucleic Acids Research·A J Marshall, L A Burgoyne
Jan 25, 1974·Science·A L Olins, D E Olins
Jul 1, 1965·Journal of Molecular Biology·J Eigner, P Doty
Sep 20, 1974·Nature·M Noll
Dec 23, 1974·Biochemical and Biophysical Research Communications·B R ShawK E Van Holde
Jun 1, 1972·The Journal of Cell Biology·D E Olins, A L Olins
Nov 1, 1974·Nucleic Acids Research·K E Van HoldeB R Shaw
Feb 1, 1965·Journal of Molecular Biology·Y MIYAZAWA, C A THOMAS

Citations

Mar 1, 1981·International Journal of Radiation Biology and Related Studies in Physics, Chemistry, and Medicine·D Suciu, O Bojan
Jun 1, 1981·International Journal of Radiation Biology and Related Studies in Physics, Chemistry, and Medicine·D Suciu
Jun 20, 2002·Transfusion Medicine·D R ChambersD Stroncek
Jan 31, 2002·Transactions of the Royal Society of Tropical Medicine and Hygiene·H EkvallA Bjorkman
Jan 1, 1985·Transactions of the Royal Society of Tropical Medicine and Hygiene·S H Abdalla, S N Wickramasinghe
Dec 1, 1987·British Journal of Haematology·S LooareesuwanD J Weatherall
Jan 1, 1987·Parasitology·P H Holmes
Jul 1, 1991·Revista Da Sociedade Brasileira De Medicina Tropical·G G Crane

Related Concepts

Calcium
Cell Nucleus
Chromatin
Cobalt Radioisotopes
DNA, Double-Stranded
Endonuclease
Hydrogen-Ion Concentration
Liver
Magnesium
NADH

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