Role of alpha v beta 5 and alpha v beta 6 integrin glycosylation in the adhesion of a colonic adenocarcinoma cell line (HT29-D4)

Journal of Cellular Biochemistry
M LehmannJ Marvaldi

Abstract

We have previously characterized the expression of the alpha nu beta 5 and alpha nu beta 6 integrins as major receptors for the human colonic adenocarcinoma cell line (HT29-D4), on vitronectin and fibronectin, respectively [Lehmann et al. (1994): Cancer Res 54:2102-2107]. In the present work we investigated the glycosylation role of these integrins in their adhesive functions. To this end, we used glycohydrolases to show that cell surface integrins were N-glycosylated and sialylated, and that only the alpha v subunit carried some immature oligosaccharide side chains. To alter the glycosylation state of the cell surface alpha v beta 5 and alpha v beta 6 integrins, we used two oligosaccharide-processing inhibitors: 1-deoxymannojirimycin (dMNJ) and tunicamycin (TM). Following treatment of HT29-D4 cells with dMNJ, cell surface alpha v beta 5 and alpha v beta 6 carried only high-mannose-type sugar chains, while TM-treated cells expressed de-N-glycosylated integrins. Neither alpha/beta heterodimers assembly nor cell surface expression were impaired in the presence of the drugs. Finally, we established that adhesion of dMNJ- or TM-treated cells was altered on both vitronectin and fibronectin substrata, whereas the adhesion of these ce...Continue Reading

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Citations

Nov 1, 2012·Biotechnology Advances·Anil KumarXing-Jie Liang
Oct 15, 2008·The Journal of Biological Chemistry·Gouri BaldwinFedor Berditchevski
Mar 30, 2001·The Journal of Biological Chemistry·J L VigneR N Taylor
Oct 31, 2000·Microscopy Research and Technique·J StutzmannP Simon-Assmann
Jun 23, 2007·International Journal of Cancer. Journal International Du Cancer·Sylvie MathieuAssou El-Battari
Jul 25, 2008·Proteomics·Anne-Sophie Vercoutter-EdouartJean-Claude Michalski

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