Role of alpha(3)beta(1) integrin in tubulogenesis of Madin-Darby canine kidney cells

Kidney International
S T JiangM J Tang

Abstract

We isolated several Madin-Darby canine kidney (MDCK) subclones that exhibit different degrees of branching tubulogenesis in lower concentrations of collagen gel. The M634 clone formed cell aggregates in 0.3% collagen gel, but developed branching tubules vigorously in 0.1% collagen gel. In contrast, the Y224 clone formed cysts in 0.3% collagen gel and displayed fewer branching structures in 0.1% collagen gel. Morphologically, M634 cells exhibited higher levels of cell scattering as well as collagen-induced cell migration than Y224. We conducted this study to delineate the underlying mechanism of branching tubulogenesis in M634 cells. Components of the focal contact machinery were analyzed in both cell lines, including the extracellular matrix glycoproteins fibronectin, laminin, and vitronectin; cytoskeleton-associated elements alpha-actinin, talin, and vinculin; and receptors for extracellular matrix and alpha(2), alpha(3), alpha(5), alpha(v), beta(1), and beta(3) integrins. Furthermore, we established several stable transfectants of alpha(3) integrin antisense RNA in M634 cells to examine the role of alpha(3)beta(1) integrin in branching morphogenesis directly. There were no obvious differences in levels of the focal adhesion c...Continue Reading

References

Jul 1, 1992·The Journal of Pathology·M A Rahilly, S Fleming
Aug 1, 1992·Biochemical Society Transactions·W H Goldmann, G Isenberg
Mar 27, 1992·Science·J M BergelsonR W Finberg
May 1, 1992·Cell·B M Gumbiner
Jun 1, 1991·American Journal of Kidney Diseases : the Official Journal of the National Kidney Foundation·P EkblomL Sorokin
Dec 2, 1990·Cell Differentiation and Development : the Official Journal of the International Society of Developmental Biologists·M E HemlerY Takada
Aug 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·P A WellerD R Critchley
Jul 1, 1987·The Journal of Cell Biology·P M Cardarelli, M D Pierschbacher
Jun 1, 1994·Cell Adhesion and Communication·N PateyL H Noel
Feb 1, 1995·Journal of Cellular Biochemistry·K TakeuchiT Irimura
May 1, 1993·American Journal of Respiratory Cell and Molecular Biology·S A MetteS M Albelda
Sep 1, 1995·Cancer Metastasis Reviews·K MatsumotoR H Kramer
Feb 1, 1996·Experimental Cell Research·S PattaramalaiA P Skubitz

❮ Previous
Next ❯

Citations

Sep 27, 2002·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Jamie A Davies
Jan 1, 2005·The Journal of Membrane Biology·Tsutomu Tsuji
Feb 24, 2006·Journal of Biomedical Science·Po-Chao ChanHong-Chen Chen
Jun 12, 2003·Methods : a Companion to Methods in Enzymology·David Cohen, Anne Müsch
Jul 29, 2008·Current Opinion in Nephrology and Hypertension·Shuta Ishibe, Lloyd G Cantley
May 12, 2010·American Journal of Physiology. Cell Physiology·Michael P Hunter, Mirjam M Zegers
May 6, 2011·American Journal of Physiology. Renal Physiology·Daniel Kai Long Tham, Hakima Moukhles
Mar 11, 2009·Journal of Biomedical Science·Wei-Chun WeiMing-Jer Tang
Feb 14, 2018·The Journal of Cell Biology·Tim B FessendenMargaret L Gardel
Jul 1, 2004·American Journal of Physiology. Renal Physiology·H A PraetoriusK R Spring
Nov 22, 2007·Journal of Cellular Biochemistry·Wei-Chun WeiMing-Jer Tang
Apr 30, 2002·Experimental Cell Research·Jeppe Praetorius, Kenneth R Spring

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.

Cell Migration

Cell migration is involved in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. Here is the latest research.