Role of alternative polyadenylation dynamics in acute myeloid leukaemia at single-cell resolution

RNA Biology
Congting YeQingshun Quinn Li

Abstract

Alternative polyadenylation (APA) has been discovered to play regulatory roles in the development of many cancer cells through preferential addition of a poly(A) tail at specific sites of pre-mRNA. A recent study found that APA was involved in the mediation of acute myeloid leukaemia (AML). However, unlike gene expression heterogeneity, little attention has been directed toward variations in single-cell APA for different cell types during AML development. Here, we used single-cell RNA-seq data of a massive population of 16,843 bone marrow mononuclear cells (BMMCs) from healthy and AML patient samples to investigate dynamic APA usage in different cell types. Abnormalities of APA dynamics in the BMMCs from AML patient samples were uncovered compared to the stable APA dynamics in samples from healthy individuals, as well as lower APA diversity between eight cell types in AML patients. Genes with APA dynamics specific to the AML samples were significantly enriched in cellular signal transduction pathways that contribute to AML development. Moreover, many leukaemic cell marker genes such as NF-κB, GATA2 and IAP-Family genes exhibited APA dynamics that specifically affected abnormal proliferation and differentiation of leukemic BMMCs...Continue Reading

References

Jun 25, 1998·Molecular and Cellular Biology·S M Jacobs-HelberS T Sawyer
Dec 1, 2001·The Journal of Biological Chemistry·Sarah M Jacobs-HelberStephen T Sawyer
Dec 14, 2002·Blood·Mustafa BenekliMeir Wetzler
Apr 28, 2007·American Journal of Respiratory and Critical Care Medicine·Stephen J ChapmanAdrian V S Hill
Feb 8, 2008·The EMBO Journal·Sven DanckwardtAndreas E Kulozik
Apr 18, 2009·Proceedings of the National Academy of Sciences of the United States of America·Zhe JiBin Tian
Jul 13, 2011·Proceedings of the National Academy of Sciences of the United States of America·Xiaohui WuArthur G Hunt
Mar 30, 2012·Omics : a Journal of Integrative Biology·Guangchuang YuQing-Yu He
Jul 10, 2012·Nucleic Acids Research·Eleonora de KlerkPeter A C 't Hoen
Jun 19, 2013·Nature Reviews. Genetics·Ran ElkonReuven Agami
Aug 7, 2013·Molecular Cancer·Bastian Hoesel, Johannes A Schmid
Sep 24, 2013·Cancer Cell·Hsu-Ping KuoMichael L Cleary
May 21, 2014·The Journal of Experimental Medicine·Andrew VolkJiwang Zhang
Jul 24, 2014·Nucleic Acids Research·Antoine-Emmanuel SalibaJörg Vogel
Dec 24, 2014·Molecular Genetics and Metabolism·Kathleen M SakamotoElizabeth A Eklund
Jun 5, 2015·Molecular Systems Biology·Lars VeltenLars M Steinmetz
Sep 17, 2015·The New England Journal of Medicine·Hartmut DöhnerClara D Bloomfield
Nov 26, 2015·Trends in Cell Biology·Christine Mayr
Dec 18, 2015·The Journal of Immunology : Official Journal of the American Association of Immunologists·Guangrui HuangAnlong Xu
Apr 16, 2016·Genome Biology·Siyuan John LiuDaniel A Lim
Apr 17, 2016·Genome Biology·Lu Wen, Fuchou Tang
Nov 4, 2016·Nature Reviews. Molecular Cell Biology·Bin Tian, James L Manley
Dec 3, 2016·Nucleic Acids Research·Minoru KanehisaKanae Morishima
Jan 17, 2017·Nature Communications·Grace X Y ZhengJason H Bielas
Aug 2, 2017·Haematologica·Iris Z UrasVeronika Sexl
Aug 9, 2017·Nature Reviews. Immunology·Efthymia Papalexi, Rahul Satija
Sep 29, 2017·The Journal of Immunology : Official Journal of the American Association of Immunologists·Feifei QiuAnlong Xu
Oct 3, 2017·Carcinogenesis·Chioniso P Masamha, Eric J Wagner
Oct 17, 2017·Genomics, Proteomics & Bioinformatics·Wei ChenTing Ni
Oct 27, 2017·PLoS Pathogens·Sylvie RatoAngela Ciuffi

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Citations

Sep 11, 2019·Nucleic Acids Research·Eldad David Shulman, Ran Elkon
Mar 18, 2020·Proteomics·Yue ZhuQiang Tian
Sep 26, 2020·Nucleic Acids Research·Federico MariniSven Danckwardt
May 10, 2020·Computational and Structural Biotechnology Journal·Congting YeQingshun Q Li
Dec 10, 2020·Biomarker Research·Fanggang RenJeffrey J Pu
Nov 6, 2020·Leukemia·Irina A Elcheva, Vladimir S Spiegelman
May 15, 2021·Trends in Molecular Medicine·Pia SommerkampAndreas Trumpp
Sep 29, 2021·Wiley Interdisciplinary Reviews. RNA·Neeraja K MohananRakesh S Laishram
Jan 21, 2022·Blood·Pia Sommerkamp, Andreas Trumpp

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Methods Mentioned

BETA
RNA-seq
scRNA-seq

Software Mentioned

SAM
bedtools
Ensembl
DB
READS
PolyA
clusterProfiler
BATSeq
Bioconductor package
Ensemble

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