Role of AMPK α in skeletal muscle glycometabolism regulation and adaptation in relation to sepsis

BioMed Research International
Xia ZhengQiang Fang

Abstract

AMP-activated protein kinase (AMPK) and the translocation of glucose transporter 4 (GLUT4) protein always involve disturbance of carbohydrate metabolism. To determine whether the change of blood glucose in the early stage of septic rat is associated with the alteration of AMPKα protein expression and GLUT4 protein translocation expression. Animal models of sepsis were induced by tail vein injection of LPS in Wistar rats. The dynamic values of blood glucose within 2 hours after injection of LPS were observed. AMPKα protein and GLUT4 protein translocation in different tissues (such as soleus muscle and extensor digitorum longus) were assessed by western blot. Blood glucose levels appeared to rise at 0.5 h after injection of LPS, arrived the peak value at 1 h, then fell at 1.5 h and 2 h Animals in LPS group experienced the increase of phos-AMPKα protein and GLUT4 protein translocation expression in soleus muscle and extensor digitorum longus. The dynamic change of blood glucose, represented in a form of initiative increase and subsequent decrease in the early stage of sepsis, may be related to glycometabolism disorder in the skeletal muscle, coming down to enhancement of GLUT4 translocation expression promoted by activation of AMPKα.

Citations

May 1, 1996·Circulation Research·W LuoE G Kranias
May 5, 1999·World Journal of Surgery·J F PatiñoJ Escallón
May 18, 1999·The American Journal of Physiology·R BergeronG I Shulman
Jan 22, 2000·The Biochemical Journal·S C SteinDavid Carling
Mar 10, 2000·Journal of Applied Physiology·E O OjukaJohn O Holloszy
Apr 18, 2001·Trends in Cell Biology·G D Holman, I V Sandoval
Jan 17, 2002·The New England Journal of Medicine·Greet Van den BergheRoger Bouillon
Feb 18, 2003·American Journal of Respiratory and Critical Care Medicine·Stefan R Bornstein, Josef Briegel
Oct 16, 2003·JAMA : the Journal of the American Medical Association·Simon J FinneyTimothy W Evans
Jan 20, 2004·Trends in Biochemical Sciences·David Carling
Jan 20, 2005·American Journal of Physiology. Endocrinology and Metabolism·David C WrightDong-Ho Han
Mar 25, 2005·Biochemical Society Transactions·L G D Fryer, David Carling
Jul 30, 2005·Intensive Care Medicine·Iain MackenzieSimon Buczaski
Oct 12, 2005·Archives of Medical Research·Gonzalo M L Bearman, Richard P Wenzel
Feb 3, 2006·The New England Journal of Medicine·Greet Van den BergheRoger Bouillon
Apr 4, 2007·Cell Metabolism·Shaohui Huang, Michael P Czech
May 17, 2007·American Journal of Physiology. Endocrinology and Metabolism·Charles H LangThomas C Vary
Dec 8, 2009·Nursing Management·Tracey Melhuish
Jun 24, 2011·Acupuncture in Medicine : Journal of the British Medical Acupuncture Society·Atsushi TominagaYoshiharu Yamamura
Dec 6, 2011·Thyroid : Official Journal of the American Thyroid Association·Erika Lia BrunettoMaria Tereza Nunes
Mar 10, 2012·Journal of Diabetes Science and Technology·Vijay Srinivasan
Sep 11, 2012·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Christudas SunilSavarimuthu Ignacimuthu
Oct 30, 2012·The Medical Clinics of North America·Romesh Khardori, Danielle Castillo
Dec 19, 2012·Shock·Rafael Simone SaiaEvelin Capellari Cárnio
Dec 5, 2013·Advances in Surgery·Patrick L Bosarge, Jeffrey D Kerby

Related Concepts

Septicemia
Western Blotting
PRKAA2 gene
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AMP-activated protein kinase alpha1 protein, rat
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Congenital, Hereditary, and Neonatal Diseases and Abnormalities
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