Role of Apollon in human melanoma resistance to antitumor agents that activate the intrinsic or the extrinsic apoptosis pathways.

Clinical Cancer Research : an Official Journal of the American Association for Cancer Research
Elena TassiAndrea Anichini

Abstract

To assess the role of Apollon in melanoma resistance to intrinsic and extrinsic pathways of apoptosis and to identify strategies to reduce its expression. Apollon expression was assessed in melanoma cells in vitro and in vivo. Apollon modulation and melanoma apoptosis were evaluated by Western blot and/or flow cytometry in response to cytotoxic drugs, mitogen-activated protein/extracellular signal-regulated kinase (MEK)-, BRAF(V600E)-, and mTOR-specific inhibitors, TRAIL and anti-HLA class II monoclonal antibodies (mAb). Mitochondrial depolarization, caspase activation, apoptosis assays, and gene expression profiling were used to test effects of Apollon silencing, by siRNA, on melanoma response to antitumor agents. Apollon was constitutively expressed by melanoma cells, in vitro and in vivo, and at higher levels than in benign melanocytic lesions. Melanoma apoptosis correlated significantly with Apollon protein downmodulation in response to cytotoxic drugs, MEK, or BRAF(V600E)-specific inhibitors. Combinatorial treatment with MEK and mTOR inhibitors and HLA class II ligation, by a specific mAb, promoted Apollon downmodulation and enhanced melanoma apoptosis. Apollon downmodulation induced by antitumor agents was caspase indepen...Continue Reading

References

Dec 14, 1999·The Journal of Investigative Dermatology·D GrossmanD C Altieri
Sep 7, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·S A DesaiS Ferrone
Aug 10, 2004·Nature Cell Biology·Yanyan HaoMikihiko Naito
Jan 11, 2005·Proceedings of the National Academy of Sciences of the United States of America·Jinyu RenChunying Du
Feb 2, 2008·Cell Death and Differentiation·B P EckelmanG S Salvesen
Feb 22, 2008·Proceedings of the National Academy of Sciences of the United States of America·James TsaiGideon Bollag
Mar 4, 2008·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Kelly A Avery-KiejdaPeter Hersey
Sep 2, 2008·Chemico-biological Interactions·Huei-Fang LiuJui-I Chao
Oct 18, 2008·The Journal of Investigative Dermatology·Thomas L HockerHensin Tsao
Oct 22, 2008·Oncogene·E C LaCasseR G Korneluk
Jun 11, 2009·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Roberta MortariniAndrea Anichini
Jun 25, 2009·Cancer Immunology, Immunotherapy : CII·Peter Hersey, Xu Dong Zhang
Jul 3, 2009·The Journal of Investigative Dermatology·Keiran S M Smalley
Oct 2, 2009·Expert Opinion on Therapeutic Targets·Koh MiuraIwao Sasaki
Jan 26, 2010·Drug Discovery Today·Raimund MannholdEmanuele Carosati
Feb 25, 2010·Cancer Research·Jens Michael SeegerHamid Kashkar
Jul 22, 2010·Cancer Research·Yongping Shao, Andrew E Aplin
Jan 22, 2011·Cancer Cell·Poulikos I Poulikakos, Neal Rosen
May 25, 2011·Nature Reviews. Clinical Oncology·Antoni Ribas, Keith T Flaherty
Jun 7, 2011·The New England Journal of Medicine·Paul B ChapmanUNKNOWN BRIM-3 Study Group

❮ Previous
Next ❯

Citations

Jan 19, 2013·Medical Oncology·Monica CantileGerardo Botti
May 30, 2013·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Paul J SmithRachel J Errington
Nov 9, 2012·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Paul J SmithRachel J Errington
Sep 10, 2014·International Journal of Cancer. Journal International Du Cancer·Wenqing TangLing Dong
Mar 25, 2014·Pigment Cell & Melanoma Research·Nethia Mohana-KumaranNikolas K Haass
Sep 22, 2015·Oncogene·V PerottiR Mortarini
Oct 27, 2017·Apoptosis : an International Journal on Programmed Cell Death·Mervat S MohamedAyat G Ali
Jul 1, 2014·Molecular & Cellular Oncology·Emilie M Charles, Markus Rehm
Jul 20, 2016·Molecular Medicine Reports·Rong LiAi-Min Leng
Oct 6, 2020·Frontiers in Cell and Developmental Biology·Qiang HeWei Lu
Apr 21, 2017·Apoptosis : an International Journal on Programmed Cell Death·Rama RathoreDietrich Büsselberg
Feb 6, 2019·Cell Death & Disease·Santiago Serrano-SaenzF Javier Oliver
Sep 25, 2016·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Iris Sze Ue LukYuzhuo Wang
Mar 7, 2021·International Journal of Molecular Sciences·Anna Makuch-KockaAnna Bogucka-Kocka

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptotic Caspases

Apoptotic caspases belong to the protease enzyme family and are known to play an essential role in inflammation and programmed cell death. Here is the latest research.

Cancer Genomics (Keystone)

Cancer genomics approaches employ high-throughput technologies to identify the complete catalog of somatic alterations that characterize the genome, transcriptome and epigenome of cohorts of tumor samples. Discover the latest research using such technologies in this feed.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Related Papers

The Journal of Investigative Dermatology
Valentina PerottiRoberta Mortarini
Clinical Cancer Research : an Official Journal of the American Association for Cancer Research
Ki Woong SungHong Hoe Koo
Zhongguo dang dai er ke za zhi = Chinese journal of contemporary pediatrics
Xiu-Hong JiaJian-Chang Li
The Journal of Heart and Lung Transplantation : the Official Publication of the International Society for Heart Transplantation
Seyedhossein AharinejadMichael Grimm
Nan fang yi ke da xue xue bao = Journal of Southern Medical University
Jian-fa ChenLi Li
© 2021 Meta ULC. All rights reserved