Abstract
It has been recognized in mammals that after pancreatic duct obstruction, acinar cells progressively disappear and pancreatic islets are preserved. Previous studies by electron microscopy have suggested the involvement of apoptosis in acinar cell deletion. In the present study, we employed molecular biological methods and investigated whether acinar cell deletion is due to apoptosis. In male Sprague-Dawley rats, pancreatic duct ligation was performed through a left paramedian incision. Pancreatic tissue was studied at each of the following intervals after ligation: 3, 6, 12, 18, and 24 h and 2, 3, 5, and 7 days. DNA fragmentation was determined by in situ labeling of DNA strand breaks on tissue sections [fluorescein-labeled terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling method (TUNEL)] and by electrophoretic detection of the fragments of extracted DNA. Tissue sections were also examined by hematoxylin/eosin staining and immunohistochemical staining of insulin. Pancreatic duct ligation induced acinar cell deletion by day 5. Pancreatic tissue from control rats demonstrated no TUNEL-positive nuclei. In contrast, acinar cells from rats 12 h to 5 days after duct ligation showed TUNEL-positive nuclei. Th...Continue Reading
Citations
Feb 24, 2001·Tissue & Cell·S TakahashiM Wakita
Sep 25, 2002·Tissue & Cell·S TakahashiM Wakita
Jul 16, 2003·Peptides·Ana M de la ManoIsabel de Dios
Jul 12, 2002·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·J-U LeeM Imamura
Feb 5, 2000·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·J U LeeM Imamura
Jun 27, 2003·Pancreas·Keisuke OkamuraHiroyuki Katoh
Jan 7, 2004·Pancreas·Guillermo GomezGeorge H Greeley
Jul 17, 2004·Pancreas·László LeindlerGyula Farkas
Nov 19, 2002·Pancreatology : Official Journal of the International Association of Pancreatology (IAP) ... [et Al.]·Hana AlgülRoland M Schmid
Aug 3, 2005·European Journal of Endocrinology·Leif JanssonArne Andersson
Jun 24, 2008·The Journal of Surgical Research·Adem KaratasHaydar Durak
Aug 3, 2004·Pancreatology : Official Journal of the International Association of Pancreatology (IAP) ... [et Al.]·Laura Iris Cosen-BinkerOsvaldo Tiscornia
Aug 1, 1997·The Journal of Surgical Research·K FujimotoM Imamura
Jun 16, 2004·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·Oliver DrognitzStefan Benz
May 16, 2007·ANZ Journal of Surgery·John C PapakostasMiltiadis I Matsagas
Nov 20, 2016·The New Phytologist·Khin Thanda WinAtsushi Yoshimura
Sep 27, 2000·American Journal of Physiology. Gastrointestinal and Liver Physiology·C R ScogginsS D Leach
Oct 28, 1998·Toxicologic Pathology·M A BreiderA W Gough
Mar 15, 2016·Journal of Toxicologic Pathology·Thomas NolteJerrold M Ward
Dec 3, 1999·International Journal of Experimental Pathology·L E Reid, N I Walker
Jul 11, 2001·Experimental Biology and Medicine·G GomezG H Greeley
Apr 5, 2003·The Journal of Laboratory and Clinical Medicine·Isabel de DiosManuel Manso