PMID: 8967336Apr 1, 1996Paper

Role of apoptotic and nonapoptotic cell death in removal of intercalated cells from developing rat kidney

The American Journal of Physiology
Jin KimK M Madsen

Abstract

In the developing rat kidney, both type A and type B intercalated cells are present throughout the medullary collecting duct (MCD), as well as the papillary surface epithelium. After birth, intercalated cells gradually disappear from the papillary surface epithelium and the terminal MCD, and type B cells disappear from the entire MCD. The purpose of this study was to establish the mechanism(s) by which intercalated cells are deleted from the MCD during development. Kidneys from 14-, 16-, 18-, and 20-day-old fetuses and 1-, 3-, 7-, and 14-day-old pups were preserved for light microscopic immunohistochemistry and electron microscopy. Intercalated cells were identified by immunostaining for H(+)-adenosinetriphosphatase (H(+)-ATPase) and band 3 protein. Apoptosis was identified by nick end labeling of DNA fragments, staining with the vital dye toluidine blue, and transmission electron microscopy. Two distinct mechanisms of elimination of intercalated cells were detected. Cells with apical labeling for H(+)-ATPase and basolateral labeling for band 3 protein protruded into the lumen of the MCD as if they were being extruded from the epithelium, and many had lost contact with the basement membrane. Extrusion of the cells with basolate...Continue Reading

Citations

Jul 19, 2003·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Tho D PhamRobert H Lane
Nov 14, 2003·American Journal of Physiology. Cell Physiology·Birgitte Mønster ChristensenSøren Nielsen
Aug 10, 2007·American Journal of Physiology. Renal Physiology·Ki-Hwan HanI David Weiner
Sep 23, 2014·American Journal of Physiology. Renal Physiology·Sunil RangarajanLisa M Curtis
Jul 12, 2002·American Journal of Physiology. Renal Physiology·Zheng ZhangMaurice B Burg
Jan 26, 2006·American Journal of Physiology. Renal Physiology·Birgitte Mønster ChristensenSøren Nielsen
Feb 22, 2001·American Journal of Physiology. Renal Physiology·C BagnisD Brown
May 22, 2003·American Journal of Physiology. Renal Physiology·Young-Hee KimKirsten M Madsen
Apr 16, 2010·American Journal of Physiology. Renal Physiology·Ki-Hwan HanI David Weiner
Aug 16, 2011·Physiology·Qais Al-Awqati, Xaio Bo Gao
Sep 15, 2007·Journal of the American Society of Nephrology : JASN·Hyun-Kuk SongJin Kim
Sep 24, 2004·Physiological Reviews·Carsten A WagnerJohn P Geibel

❮ Previous
Next ❯

Related Concepts

Related Feeds

ATP Synthases

ATP synthases are enzymes located in the inner mitochondrial membrane that catalyze the synthesis of ATP during cellular respiration. Discover the latest research on ATP synthases here.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

© 2021 Meta ULC. All rights reserved