PMID: 9551089Apr 29, 1998Paper

Role of arginine 86 of the insulin receptor in insulin binding and activation of glucose transport

Biochimica Et Biophysica Acta
N LongoM J Still

Abstract

Mutations in the insulin receptor gene cause the inherited insulin resistant syndrome leprechaunism. Patient Atl-1 with leprechaunism was homozygous for the substitution of Arg-86 with Pro (R86P) in the alpha subunit of the insulin receptor. Fibroblasts homozygous for the mutant receptor had defective insulin binding, but increased glucose transport and receptor kinase activity. The R86P mutation is located in a putative beta turn N-terminal to a proposed insulin binding domain of the receptor [P. DeMeyts, J.L. Gu, R.M. Shymko, B.E. Kaplan, G.I. Bell, J. Whittaker, Mol. Endocrinol. 4 (1990) 409-416]. To get further insight into the mechanism of the paradoxical activation of receptor signalling by the R86P mutation, the codons for proline, alanine, and glycine were substituted in the R86 position of the insulin receptor cDNA by PCR-mediated mutagenesis and stably transfected into Chinese hamster ovary (CHO) cells. Insulin binding increased 10-20 fold in CHO cells transfected with the wild type, the R86A, and the R86G insulin receptor cDNA, but did not increase in cells expressing the R86P mutation. The R86P mutation caused a constitutive activation of insulin receptor phosphorylation in CHO cells, but did not increase basal gluc...Continue Reading

References

Feb 1, 1975·Analytical Biochemistry·C Wang, R L Smith
Feb 17, 1992·Biochimica Et Biophysica Acta·N LongoL J Elsas
Apr 19, 1990·Nature·D H JonesB H Howard
Sep 18, 1987·Science·O M Rosen
Jul 1, 1989·Metabolism: Clinical and Experimental·N LongoL J Elsas
Nov 30, 1988·Biochemical and Biophysical Research Communications·C C YipI D Goldfine
May 1, 1988·The American Journal of Physiology·N LongoL J Elsas
Feb 1, 1987·Biochemical Society Transactions·K SiddleR Taylor
Aug 1, 1987·Molecular and Cellular Biology·C Chen, H Okayama
Sep 15, 1995·The Journal of Biological Chemistry·J NakaeK Fujieda
Sep 14, 1995·Nature·X J SunM F White
Jan 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·N LongoL J Elsas

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Citations

Jun 26, 2009·Cellular and Molecular Life Sciences : CMLS·Robert Root-Bernstein, Jessica Vonck
Jul 6, 2010·American Journal of Medical Genetics. Part a·Orly ArdonNicola Longo
Sep 10, 2002·American Journal of Physiology. Endocrinology and Metabolism·Mary M NellisDean J Danner

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