PMID: 9188743Jun 1, 1997Paper

Role of aromatic amino acids in carbohydrate binding of plant lectins: laser photo chemically induced dynamic nuclear polarization study of hevein domain-containing lectins

Proteins
H C SiebertH J Gabius

Abstract

Carbohydrate recognition by lectins often involves the side chains of tyrosine, tryptophan, and histidine residues. These moieties are able to produce chemically induced dynamic nuclear polarization (CIDNP) signals after laser irradiation in the presence of a suitable radical pair-generating dye. Elicitation of such a response in proteins implies accessibility of the respective groups to the light-absorbing dye. In principle, this technique is suitable to monitor surface properties of a receptor and the effect of ligand binding if CIDNP-reactive amino acids are affected. The application of this method in glycosciences can provide insights into the protein-carbohydrate interaction process, as illustrated in this initial study. It focuses on a series of N-acetylglucosamine-binding plant lectins of increasing structural complexity (hevein, pseudohevein, Urtica dioica agglutinin and wheat germ agglutinin and its domain B), for which structural NMR- or X-ray crystallographic data permit a decision of the validity of the CIDNP method-derived conclusions. On the other hand, the CIDNP data presented in this study can be used for a rating of our molecular models of hevein, pseudohevein, and domain B obtained by various modeling techniqu...Continue Reading

References

Dec 15, 1992·Carbohydrate Research·G MuralikrishnaR Schauer
Jan 1, 1991·The Plant Cell·M J Chrispeels, N V Raikhel
Oct 21, 1991·FEBS Letters·A Rodríguez-RomeroM Soriano-García
Mar 7, 1991·Biochimica Et Biophysica Acta·H J Gabius
Oct 13, 1989·Science·N Sharon, H Lis
Oct 5, 1989·Journal of Molecular Biology·C S Wright
Apr 1, 1988·Protein Engineering·W F van Gunsteren
Aug 15, 1986·Archives of Biochemistry and Biophysics·N ShibuyaW F Broekaert
Feb 14, 1971·Journal of Molecular Biology·B Lee, F M Richards
Jan 1, 1984·Journal of Molecular Evolution·H T WrightC S Wright
Sep 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·F BuckK Beyreuther
Oct 1, 1995·Plant Physiology·W J Peumans, E J Van Damme
Mar 20, 1995·FEBS Letters·K HomN Shine
Nov 16, 1994·Biochimica Et Biophysica Acta·U M SoedjanaatmadjaJ J Beintema
Apr 1, 1996·Nature Structural Biology·A K ShriveT J Greenhough
Feb 1, 1997·European Journal of Biochemistry·H J Gabius

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Citations

Jun 3, 2004·Chembiochem : a European Journal of Chemical Biology·Hans-Joachim GabiusHarold Rüdiger
Jun 19, 2016·Biochimie·Karine BerthelotSophie Lecomte
Dec 5, 2009·Journal of the American Chemical Society·Anirban BhuniaHans-Christian Siebert
Jul 16, 2004·Organic & Biomolecular Chemistry·Nuria AboitizJesús Jiménez-Barbero
Jul 7, 2009·Carbohydrate Research·Hans-Christian SiebertTibor Kozár

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