Abstract
Aryl hydrocarbon receptor nuclear translocator (ARNT) has been known to participate in cellular responses to xenobiotic and hypoxic stresses, as a common partner of aryl hydrocarbon receptor and hypoxia inducible factor-1/2alpha. Recently, it was reported that ARNT is essential for adequate insulin secretion in response to glucose input and that its expression is downregulated in the pancreatic islets of diabetic patients. In the present study, the authors addressed the mechanism by which ARNT regulates insulin secretion in the INS-1 insulinoma cell line. In ARNT knock-down cells, basal insulin release was elevated, but insulin secretion was not further stimulated by a high-glucose challenge. Electrophysiological analyses revealed that glucose-dependent membrane depolarization was impaired in these cells. Furthermore, K(ATP) channel activity and expression were reduced. Of two K(ATP) channel subunits, Kir6.2 was found to be positively regulated by ARNT at the mRNA and protein levels. Based on these results, the authors suggest that ARNT expresses K(ATP) channel and by so doing regulates glucose-dependent insulin secretion.
References
May 22, 1992·Science·H ReyesO Hankinson
Jan 20, 1995·The Journal of Biological Chemistry·G L Wang, G L Semenza
Mar 14, 1995·Proceedings of the National Academy of Sciences of the United States of America·K SogawaY Fujii-Kuriyama
Aug 30, 1996·The Journal of Biological Chemistry·H LiJ P Whitlock
Dec 1, 1996·Nature Medicine·C KaneM J Dunne
Feb 14, 1997·The Journal of Biological Chemistry·M R ProbstO Hankinson
Apr 29, 1997·Proceedings of the National Academy of Sciences of the United States of America·M EmaY Fujii-Kuriyama
Jan 10, 1998·The Journal of General Physiology·S Shyng, C G Nichols
Sep 2, 1998·Proceedings of the National Academy of Sciences of the United States of America·T MikiS Seino
Mar 5, 2002·The Journal of Biological Chemistry·Haiyan WangClaes B Wollheim
Apr 3, 2003·Journal of the National Cancer Institute·Eun-Jin YeoJong-Wan Park
Oct 14, 2004·Nucleic Acids Research·Kyoung-Hwa LeeYang-Sook Chun
Dec 8, 2004·Human Mutation·Ornella MassaUNKNOWN Early Onset Diabetes Study Group of the Italian Society of Pediatric Endocrinology and Diabetology
Apr 1, 2005·BMC Bioinformatics·Voichita D MarinescuAlberto Riva
Aug 13, 2005·Cell·Jenny E GuntonC Ronald Kahn
Oct 9, 2007·Diabetes/metabolism Research and Reviews·Osama AlsmadiBrian F Meyer
Citations
Jun 12, 2014·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Andrew J Murray, Hugh E Montgomery
Sep 28, 2015·Diabetologia·Renjitha PillaiJamie W Joseph
Dec 17, 2014·Trends in Endocrinology and Metabolism : TEM·Paul V Sabatini, Francis C Lynn
Jan 14, 2020·Biochimica Et Biophysica Acta. Molecular Cell Research·Alban GiraultHalima Ouadid-Ahidouch
Jun 19, 2021·Pflügers Archiv : European journal of physiology·Hae Young Yoo, Sung Joon Kim