PMID: 9537833Apr 16, 1998Paper

Role of astrocyte-derived tissue-type plasminogen activator in the regulation of endotoxin-stimulated nitric oxide production by microglial cells

Glia
V A VincentA M Van Dam

Abstract

In mixed glial cell cultures from cerebral cortices of newborn rats, endotoxin induces nitric oxide (NO) production in microglial cells. Earlier we demonstrated that endotoxin induced NO production by microglial cells is inhibited in the presence of astroglial cells by transforming growth factor beta (TGFbeta). Both microglial and astroglial cells produce TGFbeta in a biologically inactive form, which can be activated by plasmin generated by plasminogen activators (PA). In the present paper we describe studies on the mechanism by which glial cells may activate inactive TGFbeta and its potential inhibitory effect on NO production by microglial cells. Inhibition of plasmin increased NO production in endotoxin-treated mixed glial cell cultures. Subsequently, antibodies against tissue-type plasminogen activator (tPA) increased NO production in endotoxin-treated mixed glial cell cultures while amiloride, an inhibitor for urokinase (uPA), had no effect. We hereby concluded that tPA is the crucial PA involved in plasmin production resulting in inhibition of NO production in mixed glial cell cultures. Zymography and Northern blot analysis of purified astroglial, microglial, and mixed glial cell cultures demonstrated that astroglial cel...Continue Reading

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Citations

Nov 2, 2005·Neurotoxicity Research·I KadiuM P Thomas
Apr 30, 1999·Progress in Neurobiology·W J StreitN A Pennell
Oct 29, 1998·Mediators of Inflammation·V A VincentA M Van Dam
Apr 4, 2002·Biological Chemistry·Katerina Akassoglou, Sidney Strickland
Oct 24, 2015·Biochimica Et Biophysica Acta·Anupriya MehraFabian Docagne
May 15, 2002·Glia·Myung-Soon YangEun-Hye Joe
Aug 7, 2003·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Gregory J del Zoppo, Takuma Mabuchi
Sep 18, 2008·Journal of Neurochemistry·Andre L SamsonRobert L Medcalf
Oct 7, 2004·Brain Research·Rommy von Bernhardi, Jaime Eugenín
Jun 2, 2001·Stroke; a Journal of Cerebral Circulation·N HosomiG J del Zoppo

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