Role of autophagy in modulating post-maturation aging of mouse oocytes

Cell Death & Disease
Fei-Hu LinJing-He Tan

Abstract

Mechanisms for post-maturation oocyte aging (PMOA) are not fully understood, and whether autophagy plays any role in PMOA is unknown. To explore the role of autophagy in PMOA, expression of autophagosomes and effects of the autophagy (macro-autophagy) activity on PMOA were observed in mouse oocytes. Oocyte activation rates and active caspase-3 levels increased continuously from 0 to 18 h of in vitro aging. While levels of microtubule-associated protein light chain 3 (LC3)-II increased up to 12 h and decreased thereafter, contents of p62 decreased from 0 to 12 h and then elevated to basal level by 18 h. However, the LC3-II/I ratio remained unchanged following aging in different media or for different times. During in vitro aging up to 12 h, upregulating autophagy with rapamycin or lithium chloride decreased activation susceptibility, cytoplasmic calcium, p62 contents, oxidative stress, caspase-3 activation and cytoplasmic fragmentation while increasing developmental competence, LC3-II contents, LC3-II/I ratio, mitochondrial membrane potential, spindle/chromosome integrity and normal cortical granule distribution. Downregulating autophagy with 3-methyladenine (3-MA) produced opposite effects on all these parameters except cytopla...Continue Reading

References

May 1, 1978·Experimental Cell Research·D G Whittingham, G Siracusa
Jul 1, 1989·Journal of Reproduction and Fertility·C L ChatotI Torres
Jul 1, 1994·Molecular and Cellular Biology·W MattenG F Vande Woude
Apr 1, 1993·Human Reproduction·N J WinstonM H Johnson
Jan 24, 2002·Biology of Reproduction·Juan J TarínAntonio Cano
Feb 14, 2002·Molecular Immunology·Gennady Ermak, Kelvin J A Davies
May 22, 2002·Biology of Reproduction·Ana Carla GordoRafael Fissore
Dec 1, 1961·The Journal of Experimental Zoology·R YANAGIMACHI, M C CHANG
Sep 28, 2004·Journal of Experimental Zoology. Part A, Comparative Experimental Biology·Guo-Cheng LanJing-He Tan
Jun 2, 2005·Nature Reviews. Molecular Cell Biology·Eric H Baehrecke
Jul 1, 2005·Biology of Reproduction·Yi-Liang MiaoJing-He Tan
Sep 28, 2005·The Journal of Cell Biology·Sovan SarkarDavid C Rubinsztein
Jul 29, 2006·Autophagy·Jayanta DebnathGuido Kroemer
Jul 6, 2007·Autophagy·Noboru Mizushima, Tamotsu Yoshimori
Jul 5, 2008·Science·Satoshi TsukamotoNoboru Mizushima
Nov 29, 2008·Biology of Reproduction·Toshifumi TakahashiHirohisa Kurachi
May 20, 2009·Toxicological Sciences : an Official Journal of the Society of Toxicology·Hongyu ZhangLiankun Sun
May 26, 2009·Reproduction : the Official Journal of the Society for the Study of Fertility·Na LiuJing-He Tan
Aug 29, 2009·Cell Death and Differentiation·S Luo, D C Rubinsztein
Jun 13, 2012·European Journal of Histochemistry : EJH·M L Escobar SánchezG H Vázquez-Nin
Jul 24, 2012·Free Radical Biology & Medicine·Lin LiSpencer B Gibson
Jun 14, 2013·Zygote : the Biology of Gametes and Early Embryos·SeungHoon LeeEimei Sato
Aug 2, 2013·Reproduction, Fertility, and Development·Bong-Seok SongKyu-Tae Chang
Aug 21, 2013·Reproduction : the Official Journal of the Society for the Study of Fertility·Tessa Lord, R John Aitken
Jan 10, 2014·Nature Reviews. Molecular Cell Biology·Guillermo MariñoGuido Kroemer
Jan 22, 2015·Arthritis & Rheumatology·Paloma López de FigueroaBeatriz Caramés
Mar 4, 2015·Scientific Reports·Jiang ZhuJing-He Tan
Mar 31, 2015·BioMed Research International·Elżbieta KaniaArkadiusz Orzechowski
Jun 6, 2015·Autophagy·Francisco Torres-QuirozChristian R Landry
Oct 16, 2015·Journal of Cancer Research and Therapeutics·Rani OjhaShrawan Kumar Singh
Dec 17, 2015·Stem Cell Research & Therapy·Shipeng DangRu-Xing Wang
Aug 4, 2016·Autophagy·Xiaoli ZhangHaoxing Xu

❮ Previous
Next ❯

Citations

Jun 27, 2020·Biology of Reproduction·Guang-Yi SunJing-He Tan
May 8, 2019·Cellular and Molecular Life Sciences : CMLS·Anil Kumar YadavShail K Chaube
Aug 15, 2020·Scientific Reports·Lais B LatorracaFabíola F Paula-Lopes
Nov 13, 2020·Cell Death & Disease·Yongzhi CaoYueran Zhao
Nov 21, 2020·Scientific Reports·Bao-Yu JiaGuo-Quan Wu
Jul 3, 2021·International Journal of Molecular Sciences·Marcelo T MouraFabíola F Paula-Lopes
Aug 10, 2021·Oxidative Medicine and Cellular Longevity·Yonghui JiangHan Zhao
Mar 8, 2019·Environmental Science & Technology·Keng Po LaiRudolf Shiu Sun Wu
Jan 15, 2022·Developmental Cell·Daniela Londoño-VásquezAhmed Z Balboula

❮ Previous
Next ❯

Methods Mentioned

BETA
electrophoresis

Software Mentioned

Leica
Pro Plus
Image

Related Concepts

Related Feeds

Cell Aging

This feed focuses on cellular aging with emphasis on mitochondria, autophagy, and metabolic processes associated with aging and longevity. Here is the latest research on cell aging.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Autophagosome

An autophagosome is the formation of double-membrane vesicles that involve numerous proteins and cytoplasmic components. These double-membrane vesicles are then terminated at the lysosome where they are degraded. Discover the latest research on autophagosomes here.

Apoptotic Caspases

Apoptotic caspases belong to the protease enzyme family and are known to play an essential role in inflammation and programmed cell death. Here is the latest research.

Autophagy & Model Organisms

Autophagy is a cellular process that allows degradation by the lysosome of cytoplasmic components such as proteins or organelles. Here is the latest research on autophagy & model organisms

Cell Aging (Keystone)

This feed focuses on cellular aging with emphasis on the mitochondria, autophagy, and metabolic processes associated with aging and longevity. Here is the latest research on cell aging.

Autophagosome

An autophagosome is the formation of double-membrane vesicles that involve numerous proteins and cytoplasmic components. These double-membrane vesicles are then terminated at the lysosome where they are degraded. Discover the latest research on autophagosomes here.