Role of autophagy in monokine induced by interferon γ (Mig) production during adenovirus-hepatitis B virus infection

Hepato-gastroenterology
Yujia XiaMei Liu

Abstract

The aim of this study was to investigate the effect of HBV-induced Mig and role of autophagy in the process. Adxsi-1.3 x HBV plasmid was constructed and identified. The three cell lines (L02, HepG2, SMMC-7721) were infected with adenovirus-HBV. HBsAg and HBeAg were assessed by electrochemiluminescence immunoassay. HBV DNA, HBx, Beclin 1 and Mig expression were detected by quantitative real-Time PCR, western blotting and ELISA. The level of autophagy was evaluated by transmission electron microscope. Human fetal liver cells and hepatocellular carcinoma cells were successfully transfected with overlength HBV genome using an adenovirus vector (Ad-HBV). Ad-HBV induced Mig production and cell autophagy through up-regulation of Beclin 1 expression. We further demonstrated that the increased autophagy extent was in association with HBV-induced Mig expression. Autophagy may be a crucial intracellular mechanism of Mig induction in response to HBV infection. The results provide new insights into the pathogenesis of HBV.

Citations

Jan 13, 2016·World Journal of Gastroenterology : WJG·Shan-Ying WuHsiao-Sheng Liu

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