Role of B7 costimulatory molecules in mediating systemic and mucosal antibody responses to attenuated Salmonella enterica serovar Typhimurium and its cloned antigen

Infection and Immunity
Carlos A GarciaSuzanne M Michalek

Abstract

The purpose of the present study was to evaluate the ability of an attenuated Salmonella enterica serovar Typhimurium vaccine strain to up-regulate B7-1 and B7-2 on antigen-presenting cells and to examine the functional roles these costimulatory molecules play in mediating immune responses to Salmonella and to an expressed cloned antigen, the saliva-binding region (SBR) of antigen I/II. In vitro stimulation of B cells (B220+), macrophages (CD11b+), and dendritic cells (CD11c+) with S. enterica serovar Typhimurium induced an up-regulation of B7-2 and, especially, B7-1 expression. The in vivo functional roles of B7-1, B7-2, and B7-1/2 were evaluated in BALB/c wild-type and B7-1, B7-2, and B7-1/2 knockout (KO) mice following intranasal immunization with the Salmonella expressing the cloned SBR. Differential requirements for B7-1 and B7-2 were observed upon primary and secondary immunizations. Compared to wild-type controls, B7-1 and B7-2 KO mice had reduced mucosal and systemic anti-Salmonella antibody responses after a single immunization, while only B7-1 KO mice exhibited suppressed anti-Salmonella antibody responses following the second immunization. Mucosal and systemic antibody responses to SBR were reduced following the prim...Continue Reading

References

Aug 1, 1994·The Journal of Experimental Medicine·K S HathcockR J Hodes
Jul 1, 1994·Immunology Today·C H JuneC B Thompson
Jan 1, 1996·Annual Review of Immunology·D J LenschowJ A Bluestone
Mar 26, 2002·Nature Reviews. Immunology·Arlene H Sharpe, Gordon J Freeman
Aug 8, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Michael MartinSuzanne M Michalek

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Citations

Oct 24, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Jamal I SaadaDon W Powell

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