Role of Bacillus thuringiensis Cry1 delta endotoxin binding in determining potency during lepidopteran larval development.

Applied and Environmental Microbiology
Androulla GillilandDavid J Ellar

Abstract

Five economically important crop pests, Manduca sexta, Pieris brassicae, Mamestra brassicae, Spodoptera exigua, and Agrotis ipsilon, were tested at two stages of larval development for susceptibility to Bacillus thuringiensis toxins Cry1Ac, Cry1Ca, Cry1J, and Cry1Ba. Bioassay results for M. sexta showed that resistance to all four Cry toxins increased from the neonate stage to the third-instar stage; the increase in resistance was most dramatic for Cry1Ac, the potency of which decreased 37-fold. More subtle increases in resistance during larval development were seen in M. brassicae for Cry1Ca and in P. brassicae for Cry1Ac and Cry1J. By contrast, the sensitivity of S. exigua did not change during development. At both larval stages, A. ipsilon was resistant to all four toxins. Because aminopeptidase N (APN) is a putative Cry1 toxin binding protein, APN activity was measured in neonate and third-instar brush border membrane vesicles (BBMV). With the exception of S. exigua, APN activity was found to be significantly lower in neonates than in third-instar larvae and thus inversely correlated with increased resistance during larval development. The binding characteristics of iodinated Cry1 toxins were determined for neonate and thir...Continue Reading

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Citations

Jun 27, 2009·Environmental Science and Pollution Research International·Christoph Then
Jan 8, 2011·Journal of Agricultural and Food Chemistry·Supaporn LikitvivatanavongSarjeet S Gill
Aug 19, 2007·Applied and Environmental Microbiology·Mélanie FortierRaynald Laprade
Nov 6, 2004·Applied and Environmental Microbiology·Susana VílchezDavid J Ellar
Dec 18, 2007·Journal of Invertebrate Pathology·Patricia Hernández-MartínezBaltasar Escriche
Mar 10, 2009·Journal of Invertebrate Pathology·Kees van Frankenhuyzen
Feb 27, 2008·Pest Management Science·Miguel Michereff-FilhoMaria Urbana C Nunes
Aug 16, 2019·Journal of the Science of Food and Agriculture·Karuna RupulaSashidhar Rao Beedu
Feb 8, 2011·Environmental Toxicology and Chemistry·Jeffrey D Wolt
Apr 5, 2003·Archives of Microbiology·Ruud A de MaagdSamir Naimov
Dec 23, 2004·Environmental Biosafety Research·Gregor SchmitzPeter Pretscher
Apr 29, 2005·Insect Biochemistry and Molecular Biology·Ping WangJie Zhang
May 6, 2008·Journal of Invertebrate Pathology·J O Silva-Werneck, D J Ellar

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