PMID: 9420274Jan 7, 1998Paper

Role of baculovirus IE2 and its RING finger in cell cycle arrest

Journal of Virology
E A Prikhod'ko, L K Miller

Abstract

The ie2 gene of Autographa californica nuclear polyhedrosis virus (AcMNPV) is known to transactivate transient expression from viral promoters in a host cell-specific manner. We report that transfection of Spodoptera frugiperda (SF-21) cells with ie2 was sufficient to arrest the cell cycle, resulting in the accumulation of enlarged cells with abnormally high DNA contents. By 72 h posttransfection, more than 50% of ie2-transfected cells had DNA contents greater than 4N. There was no evidence of mitotic spindle formation in these cells, and expression of ie2 appeared to block cell cycle progression in S phase. Several ie2 mutants were analyzed to further define the region of IE2 responsible for arresting the cell cycle. Analysis of these mutants showed that deletion of the RING finger motif eliminated the ability of IE2 to arrest the cell cycle but did not affect its ability to transactivate the ie1 promoter. Moreover, mutation of a single conserved cysteine (C251) of the RING finger motif abolished the ability of IE2 to block cell cycle progression but had no apparent effect on its transregulatory activity. In contrast, a mutant of IE2 containing a deletion of residues 94 to 173 was able to block cell division but lacked trans-r...Continue Reading

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Citations

Sep 16, 1998·Proceedings of the National Academy of Sciences of the United States of America·M BelyavskyiM D Summers
Aug 21, 2002·Journal of Virology·Erin A Crouch, A Lorena Passarelli
Dec 28, 2002·Journal of Virology·Noriko ImaiWonKyung Kang
Dec 11, 2002·European Journal of Biochemistry·Kathleen L Hefferon, Lois K Miller
Jul 21, 2004·Journal of Molecular Microbiology and Biotechnology·Kathleen L Hefferon
Jan 13, 2015·The Journal of General Virology·Daniel M P Ardisson-AraujoRollie J Clem
Oct 5, 2012·Journal of Virology·Jonathan K Mitchell, Paul D Friesen
Jul 10, 1999·Journal of Virology·E A Prikhod'ko, L K Miller
Dec 5, 2018·Frontiers in Cellular and Infection Microbiology·Ying FanRoberto Bruzzone
Jan 9, 1999·Journal of Virology·G G Prikhod'koL K Miller
Feb 11, 1999·Journal of Virology·E A Prikhod'koL K Miller

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