PMID: 6170312Aug 4, 1981Paper

Role of beta2-microglobulin in the intracellular processing of HLA antigens

Biochemistry
K SegeP A Peterson

Abstract

The biosynthesis of HLA-A, -B, and -C antigens was examined in the two lymphoblastoid cell lines DAUDI and RAJI. In RAJI cells the HLA-A, -B, and -C antigen heavy chains become core-glycosylated in the endoplasmic reticulum as evidenced by their sensitivity to endo-H digestion and tunicamycin treatment. Beta2-Microglobulin is present in excess in the endoplasmic reticulum of the RAJI cells and associates with the heavy chain at the time of synthesis of the heavy chain. Pulse-chase experiments demonstrated that the RAJI HLA-A, -B, and -C antigen heavy chains become terminally glycosylated since their changed characteristics included resistance to endo-H digestion, sensitivity to neuraminidase treatment, and incorporation fucose. DAUDI HLA-A, -B, and -C antigen heavy chains are synthesized normally and become core-glycosylated but not terminally glycosylated. Other glycosylated cell surface proteins, like the HLA-DR antigens, display normal glycosylation in DAUDI cells. Therefore it is unlikely that the absence of terminally glycosylated HLA-A, -B, and -C antigen heavy chains is the result of a general defect in the biosynthetic machinery of DAUDI cells. However, DAUDI cells lack the ability to synthesize beta2-microglobulin, the...Continue Reading

References

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Citations

Jan 1, 1991·Journal of Electron Microscopy Technique·A R Beaudoin, G Grondin
Jan 1, 1989·Immunogenetics·J AlexanderP Cresswell
Jan 1, 1987·Immunologic Research·R A ZeffG Pfaffenbach
Dec 1, 1984·Cell Differentiation·S Ostrand-RosenbergM Schwartzman
Dec 1, 1986·Molecular Immunology·K SkjødtM Simonsen
Jun 1, 1992·Immunology Letters·L PaczekA Heidland
Jun 24, 1985·Biochimica Et Biophysica Acta·U FuhrmannH Ploegh
Mar 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·K WimanL Rask
Dec 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·L ClaessonP A Peterson
Dec 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·S PääboP A Peterson
Feb 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·J MiyazakiK Ozato
Oct 1, 1986·The Journal of Cell Biology·C S CopelandA Helenius
Dec 1, 1992·The Journal of Experimental Medicine·B CatipovićJ P Schneck
Oct 1, 1991·Alcoholism, Clinical and Experimental Research·J M JonesE Mezey
Mar 4, 1997·Proceedings of the National Academy of Sciences of the United States of America·E A HughesP Cresswell
Apr 1, 1984·Food Additives and Contaminants·P E Fournier, G Thomas
Sep 1, 1991·European Journal of Immunology·E K BikoffE J Robertson
May 1, 1985·European Journal of Immunology·B AxelssonP Perlmann
Jan 1, 1984·Scandinavian Journal of Immunology·K GustafssonP A Peterson

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