PMID: 9423848Jan 10, 1998Paper

Role of BfpF, a member of the PilT family of putative nucleotide-binding proteins, in type IV pilus biogenesis and in interactions between enteropathogenic Escherichia coli and host cells

Infection and Immunity
Ravi P AnanthaM S Donnenberg

Abstract

Adherence of enteropathogenic Escherichia coli (EPEC) to epithelial cells is dependent on a type IV fimbria, termed the bundle-forming pilus (BFP). A cluster of 14 genes is required for expression of BFP. The eighth gene in the cluster, bfpF, encodes a putative nucleotide-binding protein which resembles the PilT protein of Pseudomonas aeruginosa. It has been proposed that PilT is required for the retraction of the P. aeruginosa pilus, which results in twitching motility. To test the role of BfpF in BFP function and EPEC pathogenesis, two different mutations were constructed in the bfpF gene, one in the cloned gene cluster in a laboratory E. coli strain and one in wild-type EPEC. Neither mutation affected prepilin synthesis, leader sequence processing, or pilus biogenesis. However, both mutations resulted in increased localized adherence. In addition, the EPEC bfpF mutant displayed increased aggregation. The EPEC bfpF mutant was not deficient in attaching and effacing activity or invasion capacity. These results suggest that BfpF decreases aggregation and adherence by EPEC but that subsequent steps in EPEC pathogenesis do not require this protein.

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Citations

May 4, 2005·The Journal of Biological Chemistry·Lynette J CrowtherMichael S Donnenberg
Sep 7, 2001·Annual Review of Microbiology·B I KazmierczakJ N Engel
Mar 10, 2001·The Journal of Clinical Investigation·B P McNamaraG Hecht
Jul 15, 2003·Clinical Microbiology Reviews·S C ClarkeP H Williams
Jun 5, 2003·Cellular Microbiology·Jean-Philippe NougayrèdeMichael S Donnenberg
May 27, 2011·Molecular Biology of the Cell·Eitan E ZahaviBenjamin Aroeti
Sep 6, 2013·Microbiology and Molecular Biology Reviews : MMBR·Stephen Melville, Lisa Craig
Aug 30, 2000·Nature·M S Donnenberg
Mar 31, 1999·Proceedings of the National Academy of Sciences of the United States of America·C PujolX Nassif
Mar 10, 2001·The Journal of Clinical Investigation·M S Donnenberg, T S Whittam
Mar 31, 2004·Molecular Microbiology·Lynette J CrowtherMichael S Donnenberg
May 12, 2006·Journal of Medical Microbiology·Romney M HylandGlen D Armstrong
Mar 27, 2012·Journal of Bacteriology·Subramaniapillai KolappanLisa Craig
Mar 23, 2013·Acta Crystallographica. Section D, Biological Crystallography·Subramaniapillai Kolappan, Lisa Craig
Aug 26, 2017·Bioelectromagnetics·María A Quiñones-PeñaCarlos A Eslava
Jan 1, 2006·EcoSal Plus·Alfredo G Torres

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