Role of Bmi-1 in regulation of ionizing irradiation-induced epithelial-mesenchymal transition and migration of breast cancer cells

PloS One
Weiwei YuanShiyong Wu

Abstract

Radiotherapy is a widely used treatment for cancer. However, recent studies suggest that ionizing radiation (IR) can promote tumor invasion and metastasis. Bmi-1, a member of the polycomb group protein family, has been observed as a regulator of oxidative stress and promotes metastasis in some tumors. But, its potential role in the metastasis induced by IR of breast cancer has not been explored. In our study, we found that increased levels of Bmi-1 were correlated to EMT of breast cancer cells. Through analyzing the EMT state and metastasis of breast cancer induced by IR, we found the metastatic potential of breast cancer cells can either be inhibited or accelerated by IR following a time-dependent pattern. Silencing Bmi-1 completely abolished the ability of the IR to alter, reduce or increase, the migration of breast cancer cells. Also, when Bmi-1 was knocked down, the effect of inhibition of PI3K/AKT signaling on EMT affected by IR was blocked. These results suggest that Bmi-1 is a key gene in regulation of EMT and migration of breast cancer cells induced by IR through activation of PI3K/AKT signaling; therefore, Bmi-1 could be a new target for inhibiting metastasis caused by IR.

References

Mar 1, 1991·Clinical & Experimental Metastasis·C F von Essen
Jan 1, 1983·Cancer Metastasis Reviews·M M Mareel
Dec 6, 2005·Nature Reviews. Cancer·Mary Helen Barcellos-HoffEric G Wright
Mar 24, 2007·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·Jae-Won JungInn-Oc Han
May 14, 2008·International Journal of Radiation Oncology, Biology, Physics·Chun-Ta LiaoTzu-Chen Yen
Jan 16, 2009·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Wassim ChatooGilbert Bernier
Dec 22, 2009·Journal of Cancer Research and Clinical Oncology·Da-wei LiZhi-hai Peng
Jun 25, 2010·PloS One·Inês Sofia ValaSusana Constantino Rosa Santos
Jun 17, 2011·Journal of Cellular Biochemistry·Liangxian CaoThomas W Davis
Nov 26, 2011·International Journal of Radiation Oncology, Biology, Physics·Yong-Chun ZhouGuo-Zhen Guo
May 19, 2012·PloS One·Shunsuke NakamuraAtsushi Iwama
Jun 8, 2012·Frontiers in Oncology·Gabriele Multhoff, Jürgen Radons
Nov 10, 2012·Critical Reviews in Clinical Laboratory Sciences·Zbigniew DarzynkiewiczDonald Wlodkowic
Jan 15, 2013·Antioxidants & Redox Signaling·Joy N KavanaghKevin M Prise
Aug 31, 2013·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Xueyan ZhangBaohui Han
Jun 9, 2014·Critical Reviews in Oncology/hematology·Coralie MoncharmontNicolas Magné

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Citations

Jan 1, 2016·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Yongsheng ZhangQiyu Jiang
Mar 30, 2016·Cellular and Molecular Life Sciences : CMLS·Marta VilaltaEdward E Graves
Nov 3, 2016·Nature Reviews. Clinical Oncology·Olga A MartinMichael P MacManus
Nov 22, 2017·Clinical & Experimental Metastasis·Benjamin J BlythOlga A Martin
May 24, 2016·International Journal of Oncology·Jing HuYukuan Feng
Nov 6, 2015·Oncotarget·E GemenetzidisI C Mackenzie
Aug 24, 2018·Molecular Medicine·Nora SundahlMarc Mareel
Dec 22, 2019·Cells·Xueqiao JiaoLixia Xiong
Apr 30, 2020·Journal of Applied Oral Science : Revista FOB·Isadora Peres KleinVinicius Coelho Carrard
Aug 28, 2020·Frontiers in Immunology·Lia WalcherUta Kossatz-Boehlert

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Methods Mentioned

BETA
flow cytometry

Software Mentioned

SPSS
CellQuest

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