Role of BMP-4 and Its Signaling Pathways in Cultured Human Melanocytes.

International Journal of Cell Biology
Hee-Young ParkBarbara A Gilchrest

Abstract

Bone Morphogenetic Protein (BMP-4) was shown to down-regulate melanogenesis, in part, by decreasing the level of tyrosinase [Yaar et al. (2006) JBC:281]. Results presented here show that BMP-4 down-regulated the protein levels of TRP-1, PKC-beta, and MCI-R. When paired cultures of human melanocytes were treated with vehicle or BMP-4 (25 ng/ml), MAPK/ERK were phosphorylated within one hour of BMP-4 treatment. Then the activated MAPK/ERK caused an acute phosphorylation of MITF, followed by proteosome-mediated degradation of MITF, the key transcription factor for melanogenic proteins [Wu et al. (2000) Gene & Development:14]. However, prolonged exposure of melanocytes to BMP-4 (up to 48 hours) caused a decrease in the level of MITF-M transcript. In addition, BMP-4 decreased the intracellular level of cAMP, the key regulator of MITF expression. These results demonstrate that BMP-4 activates MAPK/ERK signaling pathway to transiently activate MITF; however, chronic treatment of BMP-4 to melanocytes causes a down-regulation of the expression of MITF, possibly in a cAMP-dependent pathway.

References

Feb 25, 1991·Nucleic Acids Research·F BeermannG Schütz
Nov 1, 1989·The Journal of Investigative Dermatology·P R Gordon, B A Gilchrest
Nov 1, 1995·The Journal of Investigative Dermatology·A E AllanB A Gilchrest
Sep 1, 1995·The Journal of Endocrinology·S D McLeodR S Mason
Aug 15, 1995·Proceedings of the National Academy of Sciences of the United States of America·B L RosenzweigK Miyazono
Feb 28, 1995·Proceedings of the National Academy of Sciences of the United States of America·Z Abdel-MalekV J Hearing
Oct 25, 1994·Proceedings of the National Academy of Sciences of the United States of America·A SuzukiN Ueno
Sep 1, 1994·Molecular and Cellular Biology·B B KoenigR A Grant
Apr 1, 1996·The Journal of Investigative Dermatology·M WintzenB A Gilchrest
Feb 27, 1996·Biochemical and Biophysical Research Communications·N FuseS Shibahara
Apr 1, 1996·Current Opinion in Cell Biology·P ten DijkeC H Heldin
Jul 11, 1998·The Journal of Biological Chemistry·E R PriceD E Fisher
Aug 12, 1998·The Journal of Cell Biology·C BertolottoR Ballotti
Aug 28, 1998·Cytokine & Growth Factor Reviews·M KawabataK Miyazono
Jan 5, 1999·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·N A PatelD R Cooper
Oct 15, 1993·Physical Review. B, Condensed Matter·N B An
May 29, 1999·The Journal of Biological Chemistry·H Y ParkB A Gilchrest
Jan 5, 2002·The Journal of Investigative Dermatology. Symposium Proceedings·S ShibaharaK Takahashi
Jan 5, 2002·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Guozhi XiaoRenny T Franceschi
Jan 29, 2002·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Shinji WakabayashiKunio Takaoka
Jun 18, 2003·Cell·Yigong Shi, Joan Massagué
Jun 24, 2003·The American Journal of Pathology·Jinyan DuDavid E Fisher
Dec 23, 2003·Cellular Signalling·Anja NoheNils O Petersen
Feb 14, 2004·The Journal of Investigative Dermatology·Hee-Young ParkBarbara A Gilchrest
May 19, 2004·The International Journal of Biochemistry & Cell Biology·Dong-Seok KimKyoung-Chan Park
Jul 15, 2004·Pigment Cell Research·Keith W Vance, Colin R Goding
Jul 15, 2004·Journal of Cell Science·Hee-Young ParkBarbara A Gilchrest

❮ Previous
Next ❯

Citations

Mar 9, 2011·PloS One·Bernadett KormosZsuzsanna Bata-Csörgo
Feb 8, 2014·Japanese Journal of Ophthalmology·Ying Liu, Ding Lin
Oct 16, 2013·The Journal of Investigative Dermatology·Christopher J LewisAndrey A Sharov
Dec 19, 2012·Dermatologic Therapy·Brian W LeeTorello M Lotti
Aug 12, 2014·Pigment Cell & Melanoma Research·Lorin WeinerJanice L Brissette
Jul 8, 2015·Proceedings of the National Academy of Sciences of the United States of America·Jennifer AlloucheChristine Baldeschi
Mar 9, 2019·Pigment Cell & Melanoma Research·Abdulnaser AlkhalilJeffrey W Shupp
Jun 3, 2020·International Journal of Molecular Sciences·Jae Woong JeongSungsoon Fang
Oct 20, 2020·Pharmacology & Therapeutics·Xiaoyu TianRutao Cui
Nov 14, 2020·Genes & Development·Nicole R InfarinatoElaine Fuchs
May 30, 2012·Journal of Cell Science·Suman K SinghDesmond J Tobin
Apr 19, 2021·Current Treatment Options in Oncology·Piotr Kraj

❮ Previous
Next ❯

Methods Mentioned

BETA
transgenic
PCR
transfection
enzyme-linked immunoblot assay

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.