Abstract
Burkholderia pseudomallei is the causative agent of melioidosis. The complete genome sequences of this pathogen have been revealed, which explain some pathogenic mechanisms. In various hostile conditions, for example, during nitrogen and amino acid starvation, bacteria can utilize alternative sigma factors such as RpoS and RpoN to modulate genes expression for their adaptation and survival. In this study, we demonstrate that mutagenesis of rpoN2, which lies on chromosome 2 of B. pseudomallei and encodes a homologue of the sigma factor RpoN, did not alter nitrogen and amino acid utilization of the bacterium. However, introduction of B. pseudomallei rpoN2 into E. coli strain deficient for rpoN restored the ability to utilize amino acids. Moreover, comparative partial proteomic analysis of the B. pseudomallei wild type and its rpoN2 isogenic mutant was performed to elucidate its amino acids utilization property which was comparable to its function found in the complementation assay. By contrast, the rpoN2 mutant exhibited decreased katE expression at the transcriptional and translational levels. Our finding indicates that B. pseudomallei RpoN2 is involved in a specific function in the regulation of catalase E expression.
References
Apr 1, 1991·Infection and Immunity·R RamphalS Lory
Dec 1, 1991·Infection and Immunity·M S Donnenberg, J B Kaper
Jan 1, 1991·Methods in Enzymology·K B Low
Jan 1, 1990·Journal of Bacteriology·P A TottenS Lory
Mar 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·K S Ishimoto, S Lory
May 1, 1989·Reviews of Infectious Diseases·A Leelarasamee, S Bovornkitti
Aug 1, 1989·Journal of Bacteriology·T KöhlerK N Timmis
Sep 1, 1989·Microbiological Reviews·S KustuD Weiss
Feb 1, 1974·Journal of Bacteriology·Y F Chang, E Adams
Dec 1, 1971·The Journal of Infectious Diseases·C HoweM Spotnitz
Jan 1, 1993·Journal of Cellular Biochemistry·B Magasanik
Dec 1, 1995·Microbiological Reviews·M J Merrick, R A Edwards
Jul 11, 1998·Journal of Bacteriology·J MichielsJ Vanderleyden
Nov 18, 1998·FEMS Microbiology Reviews·M M Wösten
May 25, 1999·BioTechniques·M F Alexeyev
Dec 20, 2000·Proceedings of the National Academy of Sciences of the United States of America·D P ZimmerS Kustu
Aug 31, 2001·Microbiology and Molecular Biology Reviews : MMBR·L Reitzer, B L Schneider
Nov 22, 2001·Journal of Bacteriology·E L HendricksonF M Ausubel
Jun 7, 2005·Journal of Bacteriology·Hiroyuki AraiYasuo Igarashi
Oct 13, 2006·Molecular and Cellular Probes·Patompon WongtrakoongateSumalee Tungpradabkul
Apr 15, 2009·Journal of Proteome Research·Yupaporn OsiriphunSumalee Tungpradabkul
Oct 1, 2011·Journal of Biomedicine & Biotechnology·Patompon WongtrakoongateSumalee Tungpradabkul
Mar 6, 2012·Microbiology and Immunology·Patompon WongtrakoongateSumalee Tungpradabkul
Citations
May 26, 2017·PloS One·Rahmatollah BeheshtiTakeru Igusa
Apr 14, 2020·Molecular Plant Pathology·Kaihuai LiFengquan Liu
Jun 17, 2018·Scientific Reports·Suporn PaksanontNarisara Chantratita
Jul 12, 2018·BioMed Research International·Taksaon DuanguraiPornpan Pumirat
Nov 13, 2021·Environmental Microbiology·Joyati DasGopaljee Jha