Abstract
We previously observed that aquaporins and glycerol facilitators exhibit different oligomeric states when studied by sedimentation on density gradients following nondenaturing detergent solubilization. To determine the domains of major intrinsic protein (MIP) family proteins involved in oligomerization, we constructed protein chimeras corresponding to the aquaporin AQPcic substituted in the loop E (including the proximal part of transmembrane domain (TM) 5) and/or the C-terminal part (including the distal part of TM 6) by the equivalent domain of the glycerol channel aquaglyceroporin (GlpF) (chimeras called AGA, AAG, and AGG). The analogous chimeras of GlpF were also constructed (chimeras GAG, GGA, and GAA). cRNA corresponding to all constructs were injected into Xenopus oocytes. AQPcic, GlpF, AAG, AGG, and GAG were targeted to plasma membranes. Water or glycerol membrane permeability measurements demonstrated that only the AAG chimera exhibited a channel function corresponding to water transport. Analysis of all proteins expressed either in oocytes or in yeast by velocity sedimentation on sucrose gradients following solubilization by 2% n-octyl glucoside indicated that only AQPcic and AAG exist in tetrameric forms. GlpF, GAG, ...Continue Reading
References
Apr 17, 1992·Science·G M PrestonP Agre
Aug 15, 1970·Nature·U K Laemmli
Jul 21, 1995·The Journal of Biological Chemistry·F BeuronJ P Rolland
Nov 1, 1993·The Journal of Cell Biology·J M VerbavatzA S Verkman
Oct 31, 1994·FEBS Letters·F CentenoP Falson
Oct 1, 1996·The Journal of Membrane Biology·J H Park, M H Saier
Nov 1, 1996·European Journal of Biochemistry·F Le CahérecJ F Hubert
Feb 1, 1997·Journal of the American Society of Nephrology : JASN·S M MuldersP M Deen
Jun 5, 1997·Nature·T WalzA Engel
Jun 5, 1997·Nature·A ChengA K Mitra
Jun 20, 1998·The Journal of Biological Chemistry·V LagréeS Deschamps
Jul 9, 1998·Protein Science : a Publication of the Protein Society·A FrogerC Delamarche
Jul 31, 1998·Pflügers Archiv : European journal of physiology·S M MuldersP M Deen
Dec 16, 1998·The Journal of Biological Chemistry·V LagréeJ F Hubert
Jan 20, 1999·Biochemistry·J C Mathai, P Agre
Mar 6, 1999·The Journal of Biological Chemistry·V LagréeI Pellerin
Dec 10, 1999·Biochemistry·M KuwaharaS Sasaki
Jan 14, 2000·Journal of Structural Biology·P BronW Haase
Feb 5, 2000·Journal of Molecular Biology·J B Heymann, A Engel
May 12, 2000·Journal of Structural Biology·G RenA K Mitra
Jul 25, 2000·BioTechniques·C Delamarche
Oct 4, 2000·Biochemistry·D M ManleyJ D O'Neil
Oct 18, 2000·Nature·K MurataY Fujiyoshi
Oct 20, 2000·Science·D FuR M Stroud
Jan 6, 2001·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·E J KamsteegC van Os
Feb 28, 2001·Proceedings of the National Academy of Sciences of the United States of America·M J Borgnia, P Agre
Jul 28, 2001·Kidney International·L DuchesneJ F Hubert
Sep 5, 2001·FEBS Letters·P NollertR M Stroud
Sep 5, 2001·FEBS Letters·F ZhuK Schulten
Nov 16, 2001·Structure·M O JensenK Schulten
Citations
Jul 17, 2007·Nature Structural & Molecular Biology·Teresa M BuckWilliam R Skach
Dec 2, 2010·The Journal of Biological Chemistry·Masahiro IkedaKenichi Ishibashi
Dec 13, 2003·The Plant Cell·Karolina FetterFrançois Chaumont
Feb 8, 2006·Applied and Environmental Microbiology·Mark J DanielsMark Yeager
Jan 1, 2014·Journal of Experimental Botany·Henrique NoronhaHernâni Gerós
Jun 12, 2014·FEBS Open Bio·Saki TakahashiMasahiro Ikeda
May 27, 2008·Experimental Eye Research·K VaradarajR T Mathias
Sep 21, 2005·Biology of the Cell·François ChaumontMark J Daniels
Apr 30, 2005·Biology of the Cell·Jean-François HubertCéline Raguénès-Nicol
Jan 10, 2012·Journal of Molecular Biology·Yan PanLars Konermann
Apr 26, 2016·Molecular Plant·Marie C BernyFrançois Chaumont
May 5, 2016·Molecular Plant·Yun-Joo YooInhwan Hwang
Aug 6, 2004·Plant & Cell Physiology·Shinobu Suga, Masayoshi Maeshima
Apr 23, 2009·Molecular Membrane Biology·Fredrik ObergKristina Hedfalk
Jan 21, 2016·The Journal of Biological Chemistry·Philip KitchenAlex C Conner
Apr 2, 2005·The Journal of Biological Chemistry·Xun Sean YuJean X Jiang
Oct 27, 2017·Biochimica Et Biophysica Acta. Biomembranes·Margareta TrefzDirk Schneider
Feb 20, 2021·Parasitology·Juan F Quintana, Mark C Field
Sep 11, 2002·Biochimica Et Biophysica Acta·Daniel ThomasChristian Delamarche
Feb 21, 2008·Biochemistry·Jamie J GalkaJoe D O'Neil
Jul 21, 2006·The Journal of Physical Chemistry. B·George PatargiasMark S P Sansom