Role of calcium-activated chloride current in regulating pulmonary vasomotor tone

The American Journal of Physiology
X J Yuan

Abstract

Many agonists induce vasoconstriction by releasing intracellularly stored Ca2+ and promoting Ca2+ influx. Activation of Ca(2+)-activated Cl- (ClCa) channels may be a critical mechanism by which a rise in intracellular free Ca2+ concentration ([Ca2+]i) causes membrane depolarization that serves to sustain the elevated [Ca2+]i and maintain vascular tone. In this study the biophysical and pharmacological properties of ClCa currents [ICKCa] were characterized in rat pulmonary artery (PA) smooth muscle cells, and their relationship to the regulation of pulmonary vascular tone was determined. When K+ currents were eliminated by using Cs(+)-containing internal solution, depolarization elicited an inward Ca2+ current followed by a time-dependent outward Cl- current that reversed near Cl- equilibrium potential. Repolarizing voltage steps produced a large inward tail Cl- current that also reversed at a potential close to Cl- equilibrium potential. Replacement of extracellular Ca2+ with Ba2+ significantly augmented the Ca2+ current but abolished the Cl- currents. The Cl- channel blocker niflumic acid (10-50 microM) diminished the time-dependent outward Cl- current and the inward tail Cl- current, decreased serotonin-induced membrane depol...Continue Reading

Citations

Dec 6, 2003·American Journal of Physiology. Lung Cellular and Molecular Physiology·Carmelle V Remillard, Jason X-J Yuan
Feb 3, 2012·Physiological Reviews·J T SylvesterJeremy P T Ward
Apr 3, 2014·Physiological Reviews·Nicoletta Pedemonte, Luis J V Galietta
Dec 21, 2000·American Journal of Physiology. Cell Physiology·R JaniakJ R Hume
Sep 16, 2005·American Journal of Physiology. Cell Physiology·Joseph R H MaubanJason X-J Yuan
Aug 11, 2005·Canadian Journal of Physiology and Pharmacology·Normand LeblancIain A Greenwood
Jun 30, 2009·The Journal of Membrane Biology·John E LinleyMichael A Gray
Jan 25, 2000·American Journal of Physiology. Lung Cellular and Molecular Physiology·S DoiP A Murray
Nov 29, 2002·American Journal of Physiology. Cell Physiology·Sok Han KangTerence K Smith
Oct 5, 2012·American Journal of Physiology. Cell Physiology·Abigail S ForrestNormand Leblanc
Jul 13, 2002·American Journal of Physiology. Lung Cellular and Molecular Physiology·Carmelle V RemillardJames S K Sham
Sep 10, 2004·American Journal of Physiology. Cell Physiology·Sohag N Saleh, Iain A Greenwood
Dec 14, 2004·Journal of Applied Physiology·Joseph R H MaubanJason X-J Yuan
Oct 17, 2018·International Journal of Molecular Sciences·Mélanie LambertFabrice Antigny
Aug 30, 2012·The Journal of Physiology·Toshio Kitazawa, Kazuyo Kitazawa
Feb 16, 2005·Annual Review of Physiology·Criss HartzellJorge Arreola
Feb 23, 2021·Pulmonary Circulation·Guogu LiuAiguo Dai

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