Role of calcium in acclimation of the cyanobacterium Synechococcus elongatus PCC 7942 to nitrogen starvation

Microbiology
Francisco LeganésFrancisca Fernández-Piñas

Abstract

A Ca2+ signal is required for the process of heterocyst differentiation in the filamentous diazotrophic cyanobacterium Anabaena sp. PCC 7120. This paper presents evidence that a transient increase in intracellular free Ca2+ is also involved in acclimation to nitrogen starvation in the unicellular non-diazotrophic cyanobacterium Synechococcus elongatus PCC 7942. The Ca2+ transient was triggered in response to nitrogen step-down or the addition of 2-oxoglutarate (2-OG), or its analogues 2,2-difluoropentanedioic acid (DFPA) and 2-methylenepentanedioic acid (2-MPA), to cells growing with combined nitrogen, suggesting that an increase in intracellular 2-OG levels precedes the Ca2+ transient. The signalling protein P(II) and the transcriptional regulator NtcA appear to be needed to trigger the signal. Suppression of the Ca2+ transient by the intracellular Ca2+ chelator N,N'-[1,2-ethanediylbis(oxy-2,1-phenylene)]bis[N-[2-[(acetyloxy)methoxy]-2-oxoethyl]]-,bis[(acetyloxy)methyl] ester (BAPTA-AM) inhibited expression of the glnB and glnN genes, which are involved in acclimation to nitrogen starvation and transcriptionally activated by NtcA. BAPTA-AM treatment partially inhibited expression of the nblA gene, which is involved in phycobil...Continue Reading

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Citations

Oct 5, 2010·Analytical and Bioanalytical Chemistry·Ana Lilia Barrán-BerdónFrancisca Fernández-Piñas
Dec 28, 2011·Journal of Experimental Botany·Simon StaelMarkus Teige
May 7, 2014·The Plant Journal : for Cell and Molecular Biology·Eleonora SenderskyRakefet Schwarz
Jul 16, 2015·The Plant Journal : for Cell and Molecular Biology·Eleonora SenderskyRakefet Schwarz
Jan 4, 2015·Cell Calcium·Delfina C DomínguezMarianna Patrauchan
Feb 28, 2014·Molecular Plant·Hironari Nomura, Takashi Shiina
Aug 6, 2013·The Science of the Total Environment·M Angeles Muñoz-MartínFrancisca Fernández-Piñas
May 4, 2016·Proceedings of the National Academy of Sciences of the United States of America·Brandon Scott Guida, Ferran Garcia-Pichel
Mar 27, 2018·The Plant Journal : for Cell and Molecular Biology·Mali LeviRakefet Schwarz
Oct 16, 2018·Environmental Microbiology·Karl Forchhammer, Rakefet Schwarz

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