Role of calcium in apoptosis of HL-60 cells induced by harringtonine

Science in China. Series C, Life Sciences
M FangS Xue

Abstract

The effect of Ca(2+) on HT-induced apoptosis in HL-60 cells was examined. Characteristics of apoptosis induced by harringtonine (HT) or campothecin (CAM) could not be abolished by extracellular calcium chelator EGTA; however, an intracellular Ca(2+) chelator BAPTA-AM could block HT or CAM-induced HL-60 cell apoptosis. Requirement of intracellular calcium for HT-induced apoptosis is further supported by the fact that intracellular Ca(2+) -depleted HL-60 cells could not undergo HT-induced apoptosis. No significant increase of intracellular Ca(2+) was found after HT treatment. By using video enhancement contrast microscopy (VEC), the dynamic changes of intracellular calcium distribution over the whole period of apoptosis in the same individual cell were detected. The results demonstrated the movement of Ca(2+) from cytosol to nucleus after initiation of apoptosis by treatment with HT.

References

Jun 1, 1990·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·D J McConkeyM Jondal
May 1, 1989·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·D J McConkeyS Orrenius

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Citations

May 18, 2010·International Journal of Cancer. Journal International Du Cancer·Richard NuccitelliPamela Nuccitelli
Feb 8, 2002·World Journal of Gastroenterology : WJG·Zhong-Ying ShenZeng Yi

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Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis