Role of calcium in exocrine pancreatic secretion. I. Calcium movements in the rabbit pancreas.

Biochimica Et Biophysica Acta
V V SchreursS L Bonting

Abstract

1. Calcium movements in the isolated rabbit pancreas and in rabbit pancreas fragments have been studied with the aid of 4 5 Ca2+. 2. Addition of 4 5 Ca2+ to the incubation medium of the isolated rabbit pancreas results in an immediate appearance of isotope in the secreted fluid reaching a constant specific activity in 30 min. The absolute activity in the secreted fluid is 30-40% of that in the incubation medium. 3. Addition of 10(-5) M carbachol after 2 h preincubation with 4 5 Ca2+ results in enzyme secretion accompanied by calcium release. There is also an increase in 4 5 Ca2+ secretion, but this is maximal 10 min after the protein and total calcium peaks. 4. Partial removal of 4 5 Ca2+ from the bathing medium, before stimulation, reduces the increase in 4 5 Ca2+ secretion nearly proportionally. 5. [3H]Mannitol, added to the bathing medium, appears in the secreted fluid and behaves upon carbachol stimulation similarly to 4 5 Ca2+. 6. Upon repeated stimulation with 10(-5) M acetylcholine, a 4 5 Ca2+ peak appears, even in virtual absence of enzyme secretion. In this case the peak coincides with a small total calcium peak. 7. Efflux studies of rabbit pancreas fragments, preloaded with 4 5 Ca2+, show a carbachol-stimulated 4 5 Ca...Continue Reading

References

Jul 12, 1974·The Journal of Membrane Biology·E M Wright, R J Pietras
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Sep 23, 1974·Biochemical and Biophysical Research Communications·J A Williams, M Lee

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Citations

Dec 1, 1981·Pflügers Archiv : European journal of physiology·O H PetersenI Findlay
Aug 29, 1977·Pflügers Archiv : European journal of physiology·N Ueda, O H Petersen
Oct 1, 1979·Research in Experimental Medicine. Zeitschrift Für Die Gesamte Experimentelle Medizin Einschliesslich Experimenteller Chirurgie·H TeufelM M Forell
Sep 24, 1981·European Journal of Pharmacology·J W JansenH Goebell
May 1, 1978·Biological Reviews of the Cambridge Philosophical Society·R M Case
Jul 1, 1985·Acta Physiologica Scandinavica·G M Roomans, X Wei
Nov 15, 1978·European Journal of Biochemistry·M Lambert, J Christophe
Oct 1, 1977·Archives internationales de physiologie et de biochimie·G Dandrifosse
Jul 25, 1984·Biochimica Et Biophysica Acta·G A KuijpersS L Bonting
Oct 20, 1982·Biochimica Et Biophysica Acta·O H Petersen
Jun 19, 1984·Biochimica Et Biophysica Acta·G A KuijpersJ F Rodrigues de Miranda
Feb 20, 1979·Biochimica Et Biophysica Acta·J W JansenS L Bonting
May 6, 2021·Physiological Reviews·Ole H PetersenShuang Peng

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