PMID: 9655603Jul 9, 1998Paper

Role of calcium in the activation of erp72 and heme oxygenase-1 expression on depletion of endoplasmic reticulum calcium stores in rat neuronal cell culture

Neuroscience Letters
T LindenW Paschen

Abstract

Endoplasmic reticulum (ER) calcium pool depletion was induced by 30 min exposure of primary neuronal cells to thapsigargin (Tg), an irreversible inhibitor of ER Ca2+-ATPase. Twelve hours later, erp72 and heme oxygenase-1 (HO-1) mRNA levels were quantified by PCR. Protein synthesis was also measured. Transient Tg exposure of neurons induced a marked rise in mRNA levels (7-fold and a 21-fold increase in erp72 and HO-1 mRNA levels; P < 0.001). Loading of neurons with the calcium chelator 1,2-bis(o-Aminophenoxy)ethane-N,N,N',N'-tetra(acetoxymethyl)ester (BAPTA-AM) prior to thapsigargin treatment had only a minor effect on the Tg-induced rise in gene expression. This small inhibitory effect may result from the severe suppression of protein synthesis caused by BAPTA-AM. The results suggest that the increase in stress gene expression induced by exposure of neurons to Tg is triggered by a decrease in ER calcium activity and not by the corresponding increase in cytoplasmic calcium activity.

References

Aug 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·R StockerB N Ames
Jan 1, 1985·Progress in Brain Research·W BodschK A Hossmann
Jan 15, 1993·Science·A VermaS H Snyder
Jul 1, 1994·The Journal of Membrane Biology·G Inesi, Y Sagara
Oct 1, 1993·Molecular and Cellular Neurosciences·M D Maines

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Citations

Dec 18, 1998·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·P G ClarkeV Castagné
Mar 17, 2005·Neuroreport·Do Kyung KimHong Sung Chun
Dec 12, 2012·Journal of Controlled Release : Official Journal of the Controlled Release Society·Hideaki NakamuraHiroshi Maeda
Jul 5, 2003·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Wulf Paschen
Jan 14, 1999·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·W Paschen, J Doutheil
Apr 8, 2003·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Wulf PaschenThorsten Mengesdorf
Sep 2, 2006·The Journal of Biological Chemistry·Mayuko AburayaTohru Mizushima
Jul 3, 2009·American Journal of Physiology. Cell Physiology·Gonzalo JorqueraNora Riveros
Sep 2, 2017·Journal of Neuroimmune Pharmacology : the Official Journal of the Society on NeuroImmune Pharmacology·Anna L SternCagla Akay-Espinoza
Sep 8, 2001·American Journal of Physiology. Cell Physiology·R B HunterS C Kandarian
Dec 4, 2019·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Zeyu WangQuan Cheng
Oct 27, 2010·Neurochemistry International·Hirokazu HaraTetsuo Adachi

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