PMID: 2124417Dec 1, 1990Paper

Role of calcium in tight junction formation between epithelial cells

The American Journal of Physiology
L Gonzalez-MariscalM Cereijido

Abstract

Upon transferring confluent monolayers of Madin-Darby canine kidney (MDCK) cells from a low-Ca2+ medium (1-5 microM) to one with 1.8 mM Ca2+ (Ca switch), tight junctions (TJs) assemble and seal, and transepithelial electrical resistance (TER) develops in 4-5 h, presumably through exocytotic fusion that incorporates junctional components to the surface membrane. In the present work we test this possibility and observe 1) that the Ca switch raises the cytosolic concentration of this ion; 2) that it also increases the membrane area by 22%; 3) that chloroquine, a drug which prevents exocytosis, blocks both the increase of surface membrane and the sealing of TJs; and 4) that if monolayers are not permanently switched to 1.8 mM Ca2+, but are subject to a 15-min pulse, cytosolic free Ca2+ concentration [( Ca2+]c) transiently increases but returns to low values (14 +/- 11 nM) and TER does not develop. Comparisons of the time course of TJ sealing with levels of [Ca2+]c, as well as the relationship between these parameters and extracellular Ca2+ levels, suggest that this ion may act from the extracellular side or in a narrow intracellular domain in the close vicinity of the plasma membrane.

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Citations

Nov 2, 2006·American Journal of Physiology. Renal Physiology·D Flores-BenítezR G Contreras
Aug 21, 2007·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Tamar ShultzYoram Altschuler
Jun 23, 2009·Annals of the New York Academy of Sciences·James M MullinMary Carmen Valenzano
Sep 3, 2010·Molecular Biology of the Cell·Nayden G Naydenov, Andrei I Ivanov
Oct 21, 2011·Molecular Biology of the Cell·C Clayton HazelettCharles Yeaman
Nov 4, 2011·American Journal of Physiology. Cell Physiology·M CereijidoI Larre
Jun 5, 2014·Pflügers Archiv : European journal of physiology·Yusuke KumaiSteve F Perry
Dec 19, 2014·Frontiers in Neuroscience·Hans-Christian BauerAndreas Traweger
Mar 18, 2015·Tissue Engineering. Part C, Methods·Esther MeloCharles James Kirkpatrick
Feb 21, 2018·Biotechnology and Bioengineering·Jose YesteIgnacio Giménez
May 24, 2018·American Journal of Physiology. Cell Physiology·Daniel PrestonBonnie L Blazer-Yost
Sep 24, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Andrea ToschiEster Grilli
Jul 1, 1996·Cell Adhesion and Communication·F Lacaz-Vieira, B Kachar
Jun 1, 1994·Journal of Endocrinological Investigation·J Schwartz, F M Perez
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Feb 26, 2004·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·Paulo Marcelo, François Lefèvre
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Sep 24, 2004·Physiological Reviews·M CereijidoL Shoshani
Jul 4, 2009·American Journal of Respiratory Cell and Molecular Biology·Nazanin R YacobiEdward D Crandall
Jun 12, 2012·Journal of Cell Science·Ahmed H K El-HashashDavid Warburton
Mar 26, 2014·Tissue Barriers·Sun-Young KookInhee Mook-Jung
Feb 15, 2019·Life Science Alliance·Pia Pernille SøgaardYoshifumi Itoh
Aug 28, 2019·International Journal of Molecular Sciences·Lorenza González-MariscalChristian Hernández-Guzmán
Jun 1, 1994·Journal of Cellular Physiology·R O StuartS K Nigam
Jun 1, 1994·Current Eye Research·K GreenD S Hull

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