PMID: 6410000Sep 1, 1983Paper

Role of calmodulin in the effect of guanyl nucleotides on rat hippocampal adenylate cyclase: involvement of adenosine and opiates

Journal of Neurochemistry
J M GirardotD M Cooper

Abstract

The adenylate cyclase activity of rat hippocampal plasma membranes can be stimulated by vasoactive intestinal polypeptide (VIP). Low concentrations (10(-9) to 19(-7) M) of 5'-guanylyl-imido diphosphate (GppNHp) evoke a transient inhibition of the enzyme, which is followed by stimulation with increasing GppNHp concentrations (10(-6) to 10(-4) M). Inclusion of Inclusion of ethyleneglycol-bis-(beta-aminoethylether)-N,N'-tetraacetic acid (EGTA) during incubation abolishes the GppNHp inhibition while preserving GppNHp activation. The stimulation induced by GppNHp is amplified by VIP, but the inhibition is unaffected. Adenosine analogs and opiates are inhibitory ligands in the presence of GTP, and their effects can be reversed by the appropriate receptor antagonists, 3-isobutyl-1-methylxanthine and naloxone. Treatment of membranes with trypsin abolishes the GppNHp-induced inhibition without affecting the GppNHp stimulation. The inhibition induced by GppNHp is also abolished by EGTA treatment followed by washing, which coincides with a reduction in the adenosine- and opiate-mediated, GTP-dependent inhibition. The GppNHp inhibition can be restored in EGTA-treated but not in trypsin-treated membranes by addition of calcium-calmodulin bu...Continue Reading

References

Jan 1, 1979·Methods in Enzymology·W J O'Sullivan, G W Smithers
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Jan 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·C O BrostromD J Wolff
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May 1, 1980·Archives of Biochemistry and Biophysics·W SchlegelM Rodbell

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Citations

Jun 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·K A AlbertP Greengard
Mar 1, 1988·Acta Physiologica Scandinavica·A Westlind-DanielssonT Bartfai
Apr 1, 1985·Journal of Neurochemistry·A Tamir, A M Tolkovsky
Apr 1, 1988·Journal of Cellular Biochemistry·D M CooperE Perez-Reyes

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