Role of Calpain in Spinal Cord Injury: Effects of Calpain and Free Radical Inhibitors(a)

Annals of the New York Academy of Sciences
N L BanikE L Hogan

Abstract

The demonstration of increased calpain activity, immunostaining, and expression at the gene (mRNA) and protein levels concomitant with ultrastructural degeneration and loss of axon and myelin proteins in lesioned cord have implicated a pivotal role for calpain in tissue destruction in spinal cord injury (SCI). Calpain, stimulated by free radicals, also mediates apoptotic cell death. These findings suggested that the use of calpain and lipid peroxidation drugs as therapeutic agents would protect cells and maintain the axon-myelin structural unit by preventing protein degradation. In order to examine this hypothesis, we treated SCI animals with calpain inhibitors (calpeptin) and/or methylprednisolone (MP), an antiinflammatory and free-radical inhibitor. SCI (40 g/cm) was induced by weight-drop, and 1 mg calpeptin or 165 mg MP/kg were given intravenously (i.v.) for 24 hours. Untreated injured animals receiving vehicle served as controls. Lesion 68-kDa and 200-kDa neurofilament proteins (NFPs) were analyzed by sodium dodecylsulfate polyarcylamide gel electrophoresis (SDS-PAGE) and chemiluminescence, and the extent of protein loss was quantitated. Loss of protein in the lesion of untreated cord amounted to 47% compared to sham contr...Continue Reading

References

Jun 1, 1978·Journal of Neurosurgery·G J DohrmannD Banks
Jan 1, 1992·Journal of Neurosurgery·E D Hall
Jul 1, 1991·Physiological Reviews·D E Croall, G N DeMartino
May 1, 1986·Journal of the Neurological Sciences·N L BanikK P Smith
May 1, 1980·Journal of Neuropathology and Experimental Neurology·N L BanikE L Hogan
Jun 1, 1983·Experimental Neurology·B T StokesG Hollinden
Dec 1, 1982·Neurochemical Research·N L BanikL J Whetstine
May 4, 1981·Brain Research·R D HappelJ D Balentine
Feb 10, 1995·The Journal of Biological Chemistry·R V DeshpandeN L Banik
May 1, 1994·Journal of Cellular Physiology·M K SquìerJ J Cohen
Jul 1, 1996·Journal of Neuropathology and Experimental Neurology·K E SaatmanT K McIntosh
Nov 25, 1997·Annals of the New York Academy of Sciences·D C ShieldsN L Banik

❮ Previous
Next ❯

Citations

Dec 30, 2014·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Pradeep KumarViness Pillay
Aug 27, 2018·Journal of Cellular Physiology·Omid Reza TamtajiZatollah Asemi
Dec 25, 2009·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Endre CzeiterJános Sándor
Dec 20, 2005·Acta neurochirurgica·A Büki, J T Povlishock
Jan 12, 2008·Naunyn-Schmiedeberg's Archives of Pharmacology·Jan MatthesStefan Herzig
Oct 9, 2002·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Taku SugawaraPak H Chan
Mar 5, 2011·Neurotherapeutics : the Journal of the American Society for Experimental NeuroTherapeutics·Swapan K RayNaren L Banik
Apr 25, 2020·Molecular Neurobiology·Hadi Abou-El-HassanIbrahim Omeis
May 25, 2002·British Journal of Haematology·Giorgio Lambertenghi DeliliersDavide Soligo
Jan 26, 2008·Neurochemical Research·Juan Carlos Corona, Ricardo Tapia
Jun 20, 2012·Journal of Neurotrauma·Krishna Kumar VeeravalliJasti S Rao
Jan 7, 2016·Child's Nervous System : ChNS : Official Journal of the International Society for Pediatric Neurosurgery·Kevser Kusat OlFerruh Yücel
Mar 19, 1999·Journal of Neuroscience Research·D C Shields, N L Banik
Nov 20, 1998·Experimental Eye Research·D C Shields, N L Banik
Sep 30, 2000·Bioorganic & Medicinal Chemistry Letters·W LubischA Möller
May 7, 2002·Bioorganic & Medicinal Chemistry Letters·W Lubisch, A Möller
Nov 1, 2003·The Spine Journal : Official Journal of the North American Spine Society·Gregory D Carlson, Carey Gorden
May 10, 2003·Brain Research. Brain Research Reviews·Swapan K RayNaren L Banik
Oct 12, 2004·Otolaryngologic Clinics of North America·Michael D Seidman, Prashant Vivek
May 25, 2005·Bioorganic & Medicinal Chemistry Letters·Kwang Seob LeeYong Sup Lee
Jul 29, 2008·Journal of Clinical Neuroscience : Official Journal of the Neurosurgical Society of Australasia·Osman AkdemirAhmet Colak

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis