Role of Capsid Anchor in the Morphogenesis of Zika Virus

Journal of Virology
Jyoti RanaOscar R Burrone

Abstract

The flavivirus capsid protein (C) is separated from the downstream premembrane (PrM) protein by a hydrophobic sequence named capsid anchor (Ca). During polyprotein processing, Ca is sequentially cleaved by the viral NS2B/NS3 protease on the cytosolic side and by signal peptidase on the luminal side of the endoplasmic reticulum (ER). To date, Ca is considered important mostly for directing translocation of PrM into the ER lumen. In this study, the role of Ca in the assembly and secretion of Zika virus was investigated using a pseudovirus-based approach. Our results show that, while Ca-mediated anchoring of C to the ER membrane is not needed for the production of infective particles, Ca expression in cis with respect to PrM is strictly required to allow proper assembly of infectious particles. Finally, we show that the presence of heterologous, but not homologous, Ca induces degradation of E through the autophagy/lysosomal pathway.IMPORTANCE The capsid anchor (Ca) is a single-pass transmembrane domain at the C terminus of the capsid protein (C) known to function as a signal for the translocation of PrM into the ER lumen. The objective of this study was to further examine the role of Ca in Zika virus life cycle, whether involved i...Continue Reading

References

May 1, 1990·The Journal of Membrane Biology·G von Heijne
Jul 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·M Lobigs
Feb 1, 1997·Journal of Virology·A A Khromykh, E G Westaway
Oct 29, 1997·Journal of Virology·K StadlerF X Heinz
Aug 3, 2001·Journal of Virology·P MaillardA Budkowska
May 30, 2003·The EMBO Journal·Ying ZhangMichael G Rossmann
Aug 15, 2003·The Journal of Biological Chemistry·Marc FroeschkeBernhard Dobberstein
Dec 13, 2003·Journal of Virology·Tracey J HarveyAlexander A Khromykh
Mar 3, 2004·The Journal of General Virology·Catherine EichwaldOscar R Burrone
Jul 2, 2005·World Journal of Gastroenterology : WJG·Soo-Ho ChoiSoon-B Hwang
Jul 5, 2005·Journal of Virology·Kevin WhitbyMichael S Diamond
Aug 12, 2006·The Journal of Biological Chemistry·Tomohiro YorimitsuDaniel J Klionsky
Apr 22, 2009·Cell Host & Microbe·Sonja WelschRalf Bartenschlager
Oct 27, 2009·The Journal of Infectious Diseases·Ravi JhaveriAnna Mae Diehl
May 11, 2010·Protein and Peptide Letters·Roland Ivanyi-Nagy, Jean-Luc Darlix
May 3, 2013·Journal of Virology·Victor A KostyuchenkoShee-Mei Lok
Dec 3, 2014·Current Opinion in Virology·Swapna Apte-SenguptaRichard J Kuhn
Jul 29, 2015·PLoS Neglected Tropical Diseases·Monica PoggianellaOscar R Burrone
May 6, 2016·Journal of Virology·Janja BlazevicKarin Stiasny
Oct 7, 2016·Science·Kimberly A DowdBarney S Graham
Jan 11, 2017·Biochemical and Biophysical Research Communications·M MossentaO R Burrone

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Citations

Dec 27, 2019·Mass Spectrometry Reviews·Aleksandra MilewskaPiotr Suder
May 31, 2020·The FEBS Journal·Taniya BhardwajRajanish Giri
Jan 12, 2021·Trends in Biochemical Sciences·Trisha R BarnardSelena M Sagan
May 22, 2021·Virology·Kumar Udit SaumyaRajanish Giri

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