Role of capsular modified heptose in the virulence of Campylobacter jejuni

Molecular Microbiology
Anthony WongCarole Creuzenet

Abstract

The Campylobacter jejuni capsular polysaccharide is important for virulence and often contains a modified heptose. In strain ATCC 700819 (a.k.a. NCTC 11168), the modified heptose branches off from the capsular backbone and is directly exposed to the environment. We reported previously that the enzymes encoded by wcaG, mlghB and mlghC are involved in heptose modification. Here, we show that inactivation of any of these genes leads to production of capsule lacking modified heptose and alters the transcription of other capsule modification genes differentially. Inactivation of mlghB or mlghC, but not of wcaG, decreased susceptibility to bile salts and abrogated invasion of intestinal cells. All mutants showed increased sensitivity to serum killing, especially wcaG::cat, and had defects in colonization and persistence in chicken intestine, but did not show significant differences in adhesion, phagocytosis and intracellular survival in murine macrophages. Together, our findings suggest that the capsular heptose modification pathway contributes to bacterial resistance against gastrointestinal host defenses and supports bacterial persistence via its role in serum resistance and invasion of intestinal cells. Our data further suggest a ...Continue Reading

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Citations

Sep 27, 2018·Human Vaccines & Immunotherapeutics·Frédéric PolyChad K Porter
Oct 16, 2019·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·Suwon KimDong Hae Shin
Jun 6, 2020·International Journal of Systematic and Evolutionary Microbiology·Samuel BloomfieldAnne Midwinter
Dec 21, 2017·The American Journal of Tropical Medicine and Hygiene·Rebecca SainatoChad K Porter

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