Role of carbonic anhydrase II in ectopic calcification.

Cardiovascular Pathology : the Official Journal of the Society for Cardiovascular Pathology
Rupak M RajacharCecilia M Giachelli

Abstract

Osteopontin (OPN) is a potent inhibitor of ectopic calcification. Previous studies suggested that, in addition to blocking apatite crystal growth, OPN promoted regression of ectopic calcification by inducing the expression of acid-generating carbonic anhydrase II (CAR2) in monocyte-derived cells. To test this hypothesis, OPN and CAR2 expression and calcification of subcutaneously implanted glutaraldehyde-fixed bovine pericardium (GFBP) were studied in CAR2 mutant mice. Consistent with previous studies in Black Swiss mice, GFBP calcified to a greater extent in OPN-deficient mice compared to wild types on the C57Bl/6 background. GFBP implanted in CAR2-deficient mice (CAR2(-/-)) were significantly more calcified than those implanted into wild-type mice (CAR2(+/+)) [37+/-5 vs. 20+/-6.5 microg Ca/mg tissue, respectively, at 30 days (P<.001), and 42+/-5 versus 20+/-4 microg Ca/mg tissue at 60 days, respectively (P<.001)]. On the other hand, OPN levels within and surrounding the implants were similar in CAR2(+/+) and CAR2(-/-) mice, suggesting that OPN expression in the absence of CAR2 was not sufficient to mitigate ectopic calcification. These results indicate that CAR2 expression is an important regulator of ectopic calcification, p...Continue Reading

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Citations

Jan 10, 2012·European Journal of Cardio-thoracic Surgery : Official Journal of the European Association for Cardio-thoracic Surgery·Cheul LeeYong Jin Kim
Feb 14, 2015·Journal of Cellular Physiology·Iman A Mohamed, Fatima Mraiche
Mar 17, 2015·Journal of Molecular and Cellular Cardiology·Rihab BoucharebPatrick Mathieu
May 18, 2012·Biotechnology and Bioengineering·James D BryersBuddy D Ratner
Dec 13, 2012·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Ling WuJinwoong Bok
Jun 28, 2015·American Journal of Physiology. Heart and Circulatory Physiology·Tiffany W ChangDavid S Wilkes

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