Role of carbonic anhydrase IX in human tumor cell growth, survival, and invasion

Cancer Research
Naomi RobertsonA L Harris

Abstract

Carbonic anhydrase IX (CAIX) is a membrane-associated carbonic anhydrase (CA), strongly induced by hypoxia. CAIX is overexpressed in a variety of tumor types and associated with increased metastasis and poor prognosis. An inhibitor of CAs, acetazolamide has been reported to inhibit invasion. We used RNA interference (RNAi) to examine the function of CAIX in MDA468 and MDA231 breast carcinoma cells, which express high levels of CAIX under hypoxia. Hypoxia-induced CA activity was completely blocked by specific RNAi (P < 0.01). RNAi-treated cells showed growth delay in dense monolayer culture and a 50% reduction in clonogenic survival under hypoxia. In the MDA468 cells, there was no effect of RNAi treatment on invasion. In a cell line that did not induce CAIX under hypoxia, RT112, we found no effect on the ability of cells transfected with CAIX to invade or migrate. Thus, CAIX plays an important role in the growth and survival of tumor cells under normoxia and hypoxia, making it a potential target for cancer therapy, but is not involved in invasion.

References

Mar 1, 1996·Clinical & Experimental Metastasis·R Martínez-ZaguilánM J Hendrix
May 5, 1998·Molecular and Cellular Biology·C SchmaltzD E Fisher
Oct 15, 1998·Proceedings of the National Academy of Sciences of the United States of America·S V IvanovM I Lerman
Feb 26, 2000·Proceedings of the National Academy of Sciences of the United States of America·S ParkkilaW S Sly
Mar 10, 2001·The American Journal of Pathology·S IvanovE J Stanbridge
Aug 16, 2001·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·S K ChiaA L Harris
Mar 20, 2003·Bioorganic & Medicinal Chemistry Letters·Daniela VulloClaudiu T Supuran
Oct 22, 2003·Experimental Cell Research·Eliska SvastováSilvia Pastoreková

❮ Previous
Next ❯

Citations

Apr 19, 2007·Cancer Metastasis Reviews·Peter Vaupel, Arnulf Mayer
Apr 7, 2007·Cancer Metastasis Reviews·Jung-whan KimChi V Dang
Apr 7, 2007·Cancer Metastasis Reviews·Pawel SwietachAdrian L Harris
Feb 18, 2011·Clinical & Translational Oncology : Official Publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico·María Rosario García CampeloLuis Miguel Antón Aparicio
Sep 19, 2012·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Ji Eun KwonJa Seung Koo
Mar 23, 2005·Bioorganic & Medicinal Chemistry Letters·Alessio InnocentiClaudiu T Supuran
Jul 20, 2012·Journal of the American Chemical Society·Keigo MizusawaItaru Hamachi
May 25, 2012·Journal of Medicinal Chemistry·Roben G GielingKaye J Williams
Mar 19, 2013·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·Nelson G Ordóñez
Aug 24, 2013·Nature Reviews. Cancer·Scott K ParksJacques Pouysségur
Dec 30, 2009·Prostate Cancer and Prostatic Diseases·L G SmythR W G Watson
Jul 1, 2009·The Journal of Biological Chemistry·Twan van den BeuckenBradly G Wouters
Apr 2, 2011·The Journal of Biological Chemistry·Ying LiSusan C Frost
Apr 29, 2010·Cancer Biotherapy & Radiopharmaceuticals·Gilles MeesChristophe Van de Wiele
Oct 18, 2012·Neuro-oncology·Martin A ProescholdtAlexander Brawanski
Feb 28, 2008·The American Journal of Surgical Pathology·Hikmat A Al-AhmadieSatish K Tickoo
Sep 14, 2011·BMC Cancer·Josiah V DungwaPramila Ramani
Jan 5, 2008·BMC Cancer·Sally JärveläHannu Haapasalo
Oct 31, 2013·Cell Communication and Signaling : CCS·Alice SantiPaolo Cirri
Feb 1, 2003·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Daniel E B SwinsonKenneth J O'Byrne
Feb 27, 2013·PloS One·Toshifumi MaruyamaRyutaro Kamijo
Mar 23, 2012·Sensors·Pedro Mestres-VenturaSoledad García Gómez de las Heras
May 16, 2013·World Journal of Gastroenterology : WJG·Li-Cheng LiuLin Chen
Aug 13, 2013·BioMed Research International·Ian F Robey, Lance A Nesbit
Jan 1, 2012·ISRN Otolaryngology·John Zenghong LiThian-Sze Wong
Jan 30, 2013·Journal of Cancer Research and Clinical Oncology·Dina SchützeVolkmar Müller
Oct 14, 2010·Molecular Membrane Biology·Oleg A AndreevYana K Reshetnyak
Sep 12, 2015·Expert Opinion on Therapeutic Targets·Francesca PerutNicola Baldini
Apr 10, 2013·Expert Opinion on Therapeutic Patents·Pedro A Colinas
Jun 4, 2011·Expert Opinion on Therapeutic Targets·Daniel StieberSimone P Niclou
Nov 14, 2008·Journal of Enzyme Inhibition and Medicinal Chemistry·Dharmarajan SriramPerumal Yogeeswari

❮ Previous
Next ❯

Related Concepts

Related Feeds

Breast Invasive Carcinoma

Invasive breast cancers indicate a spread into breast tissues and lymph nodes. Here are the latest discoveries pertaining to breast invasive carcinomas.

Breast Invasive Carcinoma (Keystone)

Invasive breast cancers indicate a spread into breast tissues and lymph nodes. Here are the latest discoveries pertaining to breast invasive carcinomas.