Role of carbonic anhydrases in skin wound healing

Experimental & Molecular Medicine
Harlan BarkerTero Ah Järvinen

Abstract

Skin wound closure occurs when keratinocytes migrate from the edge of the wound and re-epithelialize the epidermis. Their migration takes place primarily before any vascularization is established, that is, under hypoxia, but relatively little is known regarding the factors that stimulate this migration. Hypoxia and an acidic environment are well-established stimuli for cancer cell migration. The carbonic anhydrases (CAs) contribute to tumor cell migration by generating an acidic environment through the conversion of carbon dioxide to bicarbonate and a proton. On this basis, we explored the possible role of CAs in tissue regeneration using mouse skin wound models. We show that the expression of mRNAs encoding CA isoforms IV and IX are increased (~25 × and 4 ×, respectively) during the wound hypoxic period (days 2-5) and that cells expressing CAs form a band-like structure beneath the migrating epidermis. RNA-Seq analysis suggested that the CA IV-specific signal in the wound is mainly derived from neutrophils. Due to the high level of induction of CA IV in the wound, we treated skin wounds locally with recombinant human CA IV enzyme. Recombinant CA IV significantly accelerated wound re-epithelialization. Thus, CA IV could contrib...Continue Reading

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Citations

Oct 5, 2017·European Biophysics Journal : EBJ·Aurelija MickevičiūtėDaumantas Matulis
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Jul 28, 2020·International Journal of Molecular Sciences·Toini PemmariTero A H Järvinen
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Jun 5, 2020·Molecular Therapy : the Journal of the American Society of Gene Therapy·Toini PemmariYanling Liao
Oct 17, 2017·Experimental Eye Research·Maria VähätupaHannele Uusitalo-Järvinen

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Datasets Mentioned

BETA
E-GEOD-60424

Methods Mentioned

BETA
PCR
biopsy
RNA-Seq

Software Mentioned

Array Express
Cufflinks
Tophat
Ensembl
Bowtie

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