Role of Carbonyl Modifications on Aging-Associated Protein Aggregation

Scientific Reports
Maya TanaseLaura Santambrogio

Abstract

Protein aggregation is a common biological phenomenon, observed in different physiological and pathological conditions. Decreased protein solubility and a tendency to aggregate is also observed during physiological aging but the causes are currently unknown. Herein we performed a biophysical separation of aging-related high molecular weight aggregates, isolated from the bone marrow and splenic cells of aging mice and followed by biochemical and mass spectrometric analysis. The analysis indicated that compared to younger mice an increase in protein post-translational carbonylation was observed. The causative role of these modifications in inducing protein misfolding and aggregation was determined by inducing carbonyl stress in young mice, which recapitulated the increased protein aggregation observed in old mice. Altogether our analysis indicates that oxidative stress-related post-translational modifications accumulate in the aging proteome and are responsible for increased protein aggregation and altered cell proteostasis.

References

Mar 16, 2000·Proceedings of the National Academy of Sciences of the United States of America·A WyttenbachD C Rubinsztein
Dec 21, 2000·Trends in Cell Biology·R R Kopito
Jun 16, 2001·Molecular Biology of the Cell·S Kamhi-NesherG Z Lederkremer
May 23, 2003·Nature·Christopher J PhielPeter S Klein
Jun 6, 2003·Current Opinion in Genetics & Development·Sarah J Shoesmith Berke, Henry L Paulson
Aug 28, 2004·Molecular Biology of the Cell·Roberta KiffinAna Maria Cuervo
Sep 9, 2005·Nature·Jay R SilveiraByron Caughey
Jul 29, 2006·Autophagy·David C RubinszteinAviva Tolkovsky
May 27, 2008·Human Molecular Genetics·Esther S P WongKah-Leong Lim
Feb 12, 2010·The Journal of Biological Chemistry·Maurizio RennaDavid C Rubinsztein
Aug 17, 2010·PLoS Biology·Della C DavidCynthia Kenyon
Oct 15, 2010·Nature Reviews. Molecular Cell Biology·Jens TyedmersBernd Bukau
Oct 23, 2010·Molecular Cell·Marcia C Haigis, Bruce A Yankner
Jan 14, 2011·Journal of Chromatography. a·Christoph JohannPierluigi Reschiglian
Jul 2, 2011·Journal of Proteomics·Elvira S CannizzoLaura Santambrogio
Jul 16, 2011·International Journal of Hyperthermia : the Official Journal of European Society for Hyperthermic Oncology, North American Hyperthermia Group·Yue Zhang, Stuart K Calderwood
Oct 20, 2011·Human Molecular Genetics·Silvia CorrochanoAbraham Acevedo-Arozena
Jul 4, 2012·Trends in Cell Biology·Susmita Kaushik, Ana Maria Cuervo
Jul 31, 2012·Cell Reports·Elvira S CannizzoLaura Santambrogio
Aug 15, 2012·Molekuliarnaia biologiia·T A ShelkovnikovaN N Ninkina
Dec 6, 2012·Nature Communications·Esther WongAna Maria Cuervo
Dec 12, 2012·Annals of Neurology·Lorraine V KaliaAnthony E Lang
Apr 11, 2013·International Journal of Endocrinology·Annunziata LapollaPietro Traldi
May 25, 2013·Journal of Proteomics·Andreas Simm
Aug 6, 2013·Acta Neuropathologica·Christiane Richter-Landsberg, Janina Leyk
Aug 8, 2013·Nature Medicine·Ralph A Nixon

❮ Previous
Next ❯

Citations

Jun 28, 2016·Journal of Biomolecular Structure & Dynamics·Zarina ArifJamal Ahmad
May 19, 2017·Skin Research and Technology : Official Journal of International Society for Bioengineering and the Skin (ISBS) [and] International Society for Digital Imaging of Skin (ISDIS) [and] International Society for Skin Imaging (ISSI)·R TangF Nadal-Wollbold
May 11, 2017·Annual Review of Biochemistry·Emily Mitchell SontagJudith Frydman
Sep 7, 2017·Antioxidants & Redox Signaling·Alexey GolubevVadim N Gladyshev
Dec 20, 2018·The Journal of Biological Chemistry·Amy K HauckDavid A Bernlohr
Mar 28, 2019·Open Biology·Anita Krisko, Miroslav Radman
Jan 26, 2020·Antioxidants·Magdalena RudzińskaAndrey A Zamyatnin
Sep 29, 2017·Prion·Marie C Lechler, Della C David
May 18, 2020·Journal of Huntington's Disease·Emily Machiela, Amber L Southwell
Jun 2, 2017·Frontiers in Aging Neuroscience·Nicole GrohDella C David
Aug 14, 2019·International Journal of Molecular Sciences·Elise LévyLaurence Vernis
Jul 10, 2020·European Respiratory Review : an Official Journal of the European Respiratory Society·Martina KorfeiSilke Meiners
Sep 13, 2017·Oxidative Medicine and Cellular Longevity·Ana L Santos, Ariel B Lindner
May 26, 2018·Skin Research and Technology : Official Journal of International Society for Bioengineering and the Skin (ISBS) [and] International Society for Digital Imaging of Skin (ISDIS) [and] International Society for Skin Imaging (ISSI)·V SamouillanA-M Schmitt
Dec 25, 2019·Evidence-based Complementary and Alternative Medicine : ECAM·Juan LiGang Chen
Sep 27, 2019·Journal of Cellular Physiology·Michael DugganJennifer Gordon
Jun 13, 2020·Frontiers in Physiology·Justin M Quiles, Åsa B Gustafsson
Nov 10, 2020·Rejuvenation Research·Shambhoo Sharan TripathiSyed Ibrahim Rizvi
Jul 1, 2020·Ageing Research Reviews·Stephany FranciscoManuel A S Santos
Mar 18, 2021·Antioxidants & Redox Signaling·Marilene DemasiLeonor Thomson
Mar 30, 2021·Ageing Research Reviews·Rohan GuptaPravir Kumar
Aug 17, 2018·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Kamel CharradiEzzedine Aouani
Jun 3, 2021·Free Radical Research·Moeri OshimoMitsugu Akagawa

❮ Previous
Next ❯

Methods Mentioned

BETA
light scattering
fluorescence imaging
carbonylation
dynamic light scattering
protein folding
ubiquitination
FACS
Assay

Software Mentioned

Mascot
Proteome
GraphPad Prism
Proteome Discoverer
Astra
Eclipse®
GraphPad
ZetaPlus Particle Sizing
Eclipse ISIS
ChemStation

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Advances in Protein Chemistry
Timothy D Veenstra
Nature Reviews. Neuroscience
Darran Yates
Antioxidants & Redox Signaling
Yuichiro J SuzukiD Allan Butterfield
© 2022 Meta ULC. All rights reserved