Role of cationic carbosilane dendrons and metallic core of functionalized gold nanoparticles in their interaction with human serum albumin

International Journal of Biological Macromolecules
Dzmitry ShcharbinMaria Bryszewska

Abstract

Functionalization of gold nanoparticles by different chemical groups is an important issue regarding the biomedical applications of such particles. Therefore we have analyzed the interaction between gold nanoparticles functionalized by carbosilane dendrons with human serum albumin at different pHs, and in the presence of the protein unfolding agent, guanidine hydrochloride, using circular dichroism, zeta-potential and fluorescence quenching. The effect of a nanoparticle dendronization and pure dendrons on the immunoreactivity of albumin was estimated using ELISA. In addition, the tool to estimate the binding capacity of dendronized gold nanoparticles using a hydrophobic fluorescent probe 1,8-ANS (1-anilinonaphthalene-8-sulfonic acid) was chosen. We concluded that the effect of a nanoparticle on the structure, immunochemical properties and unfolding of albumin significantly decreased with second and third generations dendrons attached. Differences in pH dependence of the interaction between nanoparticles, their dendrons and albumin showed several effects of the "dendritic corona" and the metallic part of nanoparticle on the protein. These interactions indicate changes in the immunoreactivity of the protein, whereas dendron coati...Continue Reading

Citations

Jul 6, 2019·Mikrochimica Acta·Romy Vásquez-VillanuevaM Concepción García
Jul 8, 2020·International Journal of Molecular Sciences·Elżbieta Pędziwiatr-WerbickaMaria Bryszewska
Jan 23, 2021·The Journal of Physical Chemistry. B·Elżbieta Pędziwiatr-WerbickaMaria Bryszewska
Mar 18, 2021·Journal of Biotechnology·Maria TerehovaDzmitry Shcharbin

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