Role of CD80 in stimulating T lymphocyte activation

World Journal of Gastroenterology : WJG
De-Chun LiJian-Xin Fu

Abstract

To observe biological characteristics of hepatocarcinoma cells before and after CD80 transfection and to compare the effect of CD80-transfected hepatocarcinoma cells on T lymphocyte activation. Retro virus vector carrying CD80 gene was transfected into HepG2 cells to establish CD80-transfected hepatocarcinoma cells (HepG2/hCD80). Flow cytometry (FCM) was performed to detect CD80 expression in the transfected cells. RT-PCR was used to evaluate CD80 expression at mRNA level. In the presence of anti-CD3 mAb, the proliferation of T lymphocyte was observed by MTT. Meanwhile, the expression of activated molecule marker CD25 was analyzed through FCM. A stable cell line HepG2/hCD80 expressing the human CD80 was established. Growth curve showed that the molecule CD80 could obviously decrease the growth of tumor cells. HepG2/hCD80 was evidenced to have a potency to enhance T cell proliferation and upregulate CD25 expression. CD80 transfection can lower malignant phenotype of hepatocarcinoma cells. CD80 transfection has a down-regulatory effect to activated T cells in vitro.

References

Jul 1, 1989·The Journal of Experimental Medicine·J H PhillipsL L Lanier
Jul 15, 1996·The Journal of Surgical Research·S Dessureault, S Gallinger
May 1, 1997·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·T TatsumiN Hayashi
Mar 29, 2001·Trends in Immunology·C A Chambers

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cancer Vaccines

Cancer vaccines are vaccines that either treat existing cancer or prevent development of a cancer.

Related Papers

Di 1 jun yi da xue xue bao = Academic journal of the first medical college of PLA
Shao-xiang LiXin-yuan Liu
Zhonghua gan zang bing za zhi = Zhonghua ganzangbing zazhi = Chinese journal of hepatology
Si-lang ZhouJi-ren Zhang
Nan fang yi ke da xue xue bao = Journal of Southern Medical University
Yi DingAi-min Sun
Zhong yao cai = Zhongyaocai = Journal of Chinese medicinal materials
Qin DuShan Deng
© 2021 Meta ULC. All rights reserved