Role of cell adhesion molecule DM-GRASP in growth and orientation of retinal ganglion cell axons

Developmental Biology
Hasan X AvciG E Pollerberg

Abstract

The cell adhesion molecule (CAM) DM-GRASP was investigated with respect to a role for axonal growth and navigation in the developing visual system. Expression analysis reveals that DM-GRASP's presence is highly spatiotemporally regulated in the chick embryo retina. It is restricted to the optic fiber layer (OFL) and shows an expression maximum in a phase when the highest number of retinal ganglion cell (RGC) axons extend. In the developing retina, axons grow between the DM-GRASP-displaying OFL and the Laminin-rich basal lamina. We show that DM-GRASP enhances RGC axon extension and growth cone size on Laminin substrate in vitro. Preference assays reveal that DM-GRASP-containing lanes guide RGC axons, partially depending on NgCAM in the axonal membrane. Inhibition of DM-GRASP in organ-cultured eyes perturbs orientation of RGC axons at the optic fissure. Instead of leaving the retina, RGC axons cross the optic fissure and grow onto the opposite side of the retina. RGC axon extension per se and navigation from the peripheral retina towards the optic fissure, however, is not affected. Our results demonstrate a role of DM-GRASP for axonal pathfinding in an early phase of the formation of the higher vertebrate central nervous system.

References

Jul 1, 1991·Trends in Biochemical Sciences·S E D'SouzaE F Plow
Jan 1, 1990·Cold Spring Harbor Symposia on Quantitative Biology·B StahlF Bonhoeffer
Aug 1, 1990·Development·O PourquiéN M Le Douarin
Jan 1, 1990·Experimental Brain Research·J VielmetterC A Stuermer
Sep 1, 1988·Brain Research·A M SheppardP L Jeffrey
Feb 1, 1987·The Journal of Cell Biology·F G RathjenU Rutishauser
Apr 1, 1986·Developmental Biology·W Halfter, S Deiss
Mar 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·S ThanosU Rutishauser
Jan 1, 1980·Methods in Enzymology·M G Mage
Jan 1, 1983·Developmental Biology·W HalfterU Schwarz
Jul 1, 1995·Experimental Neurology·R L Snow, J A Robson
May 1, 1996·The Journal of Cell Biology·A P DeBernardo, S Chang
Jan 29, 1999·The Journal of Cell Biology·C A LeppertC A Stuermer
Aug 6, 1999·Developmental Biology·J P StephanJ P Mather
Dec 23, 1999·The Journal of Biological Chemistry·A W SchaeferV Lemmon
Apr 18, 2001·The Journal of Biological Chemistry·L C van KempenG W Swart
Jun 26, 2001·Brain Research. Brain Research Reviews·S Thanos, J Mey
Jan 1, 1992·The European Journal of Neuroscience·W. Halfter, Y. Von Boxberg

❮ Previous
Next ❯

Citations

Jan 23, 2013·Molecular Neurobiology·Laura CampelloJosé Martín-Nieto
Jan 1, 2011·The AFCS-nature Molecule Pages·Amanda G HansenAndries Zijlstra
Dec 26, 2015·Biology·Mirka HomrichSimone Diestel
Aug 6, 2011·The Journal of Surgical Research·Michael TachezyMaximilian Bockhorn
Jan 17, 2012·Journal of Neurochemistry·Anna WadeGiampietro Schiavo
Nov 28, 2012·Mechanisms of Development·G E PollerbergB C Hochlehnert
Dec 17, 2009·Developmental Biology·Katherine E BrownJoachim Wittbrodt
Sep 29, 2009·Developmental Biology·Hannah ThompsonLynda Erskine
May 24, 2016·Neural Regeneration Research·Shruti Jain, Kristy Welshhans
Dec 20, 2012·PloS One·Karsten ThelenG Elisabeth Pollerberg
Dec 25, 2008·The Journal of Comparative Neurology·Heike Diekmann, Claudia A O Stuermer
Feb 8, 2007·Journal of Experimental Zoology. Part B, Molecular and Developmental Evolution·Mark S Cooper, Valerie C Virta
Mar 17, 2012·Journal of Cell Science·Karsten ThelenG Elisabeth Pollerberg

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cell Adhesion Molecules in the Brain

Cell adhesion molecules found on cell surface help cells bind with other cells or the extracellular matrix to maintain structure and function. Here is the latest research on their role in the brain.

Brain developing: Influences & Outcomes

This feed focuses on influences that affect the developing brain including genetics, fetal development, prenatal care, and gene-environment interactions. Here is the latest research in this field.

Advanced Imaging of Cellular Signaling

Cell signaling is a vital mechanism for communication within cells and outside with the environment. Several different signaling pathways have been found and advanced imaging techniques are being developed to visualize the molecules involved in these signaling pathways. Find the latest research in advanced imaging of cellular signaling here.

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.