Role of β Cell Precursors in the Regeneration of Insulin-Producing Pancreatic β Cells under the Influence of Glucagon-Like Peptide 1

Bulletin of Experimental Biology and Medicine
E G SkurikhinA M Dygai

Abstract

The effects of the pegylated form of glucagon-like peptide 1 (pegGLP-1) on oligopotent β cell precursors (CD45-TER119-CD133+CD49flow) in the pancreas were studied in C57Bl/6 mice. Under conditions of streptozotocin-induced type 1 diabetes mellitus, intraperitoneal injection of pegGLP1 increased the content of β cell precursors and dithizone-stained cells in the pancreas. β Cell precursors of mice with diabetes demonstrated high self-maintenance potential. In contrast to pegGLP-1, native GLP-1 did not affect β cell precursors in diabetic animals. Treatment of a culture of β cell precursors from mice with diabetes induced the yield of dithizone-stained mononuclears. In conditioned mediums of dithizone-positive cells obtained as a result of differentiation of β cell precursors from mice with diabetes, insulin was detected after administration of pegGLP-1 (10-7 M) and glucose (3 mmol/liter); the level of insulin increased with increasing glucose concentration (to 20 mmol/liter). The in vitro effect of pegGLP-1 did not differ from the effect of GLP-1 (10-7 M).

References

Dec 22, 1999·Endocrine Reviews·T J Kieffer, J F Habener
Aug 24, 2006·American Journal of Physiology. Endocrinology and Metabolism·Masaaki OkunoSusumu Seino
May 16, 2014·Bulletin of Experimental Biology and Medicine·E G SkurikhinA M Dygai

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Citations

Mar 2, 2019·Diabetology & Metabolic Syndrome·Mairim Alexandra SolisLynn L H Huang
Feb 8, 2020·International Journal of Molecular Sciences·Angelina Vladimirovna PakhomovaEvgenii Germanovich Skurikhin

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