PMID: 9545567May 16, 1998Paper

Role of cholesterol in the modulation of interdigitation in phosphatidylethanols

Biochimica Et Biophysica Acta
O P Bondar, E S Rowe

Abstract

Phosphatidylethanol (Peth) is formed in biological membranes when ethanol replaces water in the transphosphatidylation reaction catalyzed by phospholipase D. This charged lipid accumulates in the presence of ethanol, and it has unusual properties that can influence membrane structure and function. We have previously shown that dimyristoylphosphatidylethanol (DMPeth) and dipalmitoylphosphatidylethanol (DPPeth) form the interdigitated gel phase in the presence of Tris-HCl [O.P. Bondar, E.S. Rowe, Biophys. J., 71 (1996) 1440-1449]. In the present investigation, differential scanning calorimetry (DSC) and fluorescence have been used to investigate the effect of cholesterol on the phase behavior of DPPeth and DMPeth. Our results show that cholesterol prevents the formation of the interdigitated phase in the presence of Tris-HCl, and that ethanol counters this influence and restores the ability of these lipids to interdigitate. Pyrene-PC fluorescence probe was used in this investigation and gave results that were in agreement with the conclusions based on the DSC study.

References

Jan 1, 1988·Progress in Lipid Research·J L Slater, C H Huang
Jun 1, 1987·Chemistry and Physics of Lipids·P LaggnerA Schuster
Jun 16, 1987·Biochemistry·J MattaiG G Shipley
Dec 11, 1987·Biochimica Et Biophysica Acta·D A WilkinsonT J McIntosh
Feb 13, 1987·Biochemical and Biophysical Research Communications·L Gustavsson, C Alling
Dec 9, 1985·Biochimica Et Biophysica Acta·P L Yeagle
Aug 28, 1984·Biochemistry·T J McIntoshN A Porter
Oct 15, 1983·Journal of Molecular Biology·E N SerrallachG G Shipley
Mar 27, 1984·Biochimica Et Biophysica Acta·C AllingE Anggård
May 22, 1995·Chemistry and Physics of Lipids·N BorochovD Bach
Sep 1, 1996·Biophysical Journal·O P Bondar, E S Rowe

❮ Previous
Next ❯

Citations

Mar 24, 2005·Colloids and Surfaces. B, Biointerfaces·Masataka KusubeShoji Kaneshina
Sep 12, 2001·Chemistry and Physics of Lipids·I WinterK Lohner
Feb 6, 2003·Biophysical Chemistry·Miroslav Karabaliev, Valery Kochev
Mar 9, 2010·Chemical Reviews·Renatus W SinkeldamYitzhak Tor
Apr 22, 2014·Biophysical Chemistry·Eric A SmithPhoebe K Dea
Oct 30, 2007·Chemistry and Physics of Lipids·Patrick GaridelKlaus Brandenburg
Apr 3, 2010·Biochimica Et Biophysica Acta·Shishir JaikishanJ Peter Slotte
Feb 18, 2004·Biochimica Et Biophysica Acta·Ioanna KyrikouGeorge Nounesis
Mar 3, 2010·The Journal of Physical Chemistry. B·Heather A Pillman, G J Blanchard
May 17, 2021·Chemistry and Physics of Lipids·Masroor Hossain, G J Blanchard
Jan 23, 1999·Biophysical Journal·O P Bondar, E S Rowe

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.