Role of chondroitin 4-sulphate as a receptor for polycation induced human platelet aggregation

British Journal of Pharmacology
J L DonatoG De Nucci

Abstract

1. Proteoglycans provide negatively charged sites on the surface of platelets, leukocytes and endothelial cells. Since chondroitin 4-sulphate is the main proteoglycan present on the platelet surface, the role of this molecule in mediating the activation of human platelets by polylysine was studied. 2. Platelets were desensitized with phorbol 12-myristate 13-acetate (PMA, 10 nM) 5 min before the addition of polylysine to platelet-rich plasma (PRP). Changes in the intracellular Ca2+ concentration were measured in fura2-am (2 microM) loaded platelets and protein phosphorylation was assessed by autoradiography of the electrophoretic profile obtained from [32P]-phosphate labelled platelets. The release of dense granule contents was measured in [14C]-5-hydroxytryptamine loaded platelets and the synthesis of thromboxane (TXA2) was assessed by radioimmunoassay. Surface chondroitin 4-sulphate proteoglycan was degraded by incubating platelets with different concentrations of chondroitinase AC (3 min, 37 degrees C). The amount of chondroitin 4-sulphate remaining in the platelets was then quantified after proteolysis and agarose gel electrophoresis. 3. The addition of PMA to PRP before polylysine inhibited the aggregation by 88 +/- 18% (n ...Continue Reading

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Citations

Dec 20, 2002·Biology of the Cell·Ramon A JorqueraJuan G Reyes
Oct 3, 2002·American Journal of Respiratory and Critical Care Medicine·Abderrahim NemmarBenoit Nemery
Jun 28, 2011·Blood·Tobias A FuchsDenisa D Wagner
Jul 23, 2013·Life Sciences·Aline Mendes MazieroGilberto De Nucci
Mar 15, 2006·Immunological Investigations·Ugur Gonlugur, Tanseli Efeoglu Gonlugur

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