Role of chromosomal and plasmid-borne receptor homologues in the response of Bacillus megaterium QM B1551 spores to germinants

Journal of Bacteriology
G Christie, Christopher R Lowe

Abstract

Spores of Bacillus megaterium QM B1551 germinate in response to a number of trigger compounds, including glucose, proline, leucine, and inorganic salts. An approximate 6-kb region of the 165-kb plasmid was found to harbor a tricistronic receptor operon, gerU, and a monocistronic receptor component, gerVB. The gerU operon was observed to complement the germination response in plasmidless strain PV361 to glucose and leucine, with KBr acting as a cogerminant. Proline recognition is conferred by the monocistronic gerVB gene, the presence of which also improves the germination response to other single-trigger compounds. A chimeric receptor, GerU*, demonstrates interchangeability between receptor components and provides evidence that it is the B protein of the receptor that determines germinant specificity. Introduction of the gerU/gerVB gene cluster to B. megaterium KM extends the range of germinants recognized by this strain to include glucose, proline, and KBr in addition to alanine and leucine. A chromosomally encoded receptor, GerA, the B component of which is predicted to be truncated, was found to be functionally redundant. Similarly, the plasmid-borne antiporter gene, grmA, identified previously as being essential for germina...Continue Reading

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Citations

Apr 30, 2013·Journal of Bacteriology·Arturo Ramirez-PeraltaPeter Setlow
Apr 30, 2013·Journal of Bacteriology·Srishti GuptaG Christie
May 13, 2008·Journal of Bacteriology·B SetlowPeter Setlow
Jan 13, 2009·Journal of Bacteriology·Sonali Ghosh, Peter Setlow
Dec 18, 2007·Journal of Bacteriology·Daniel Paredes-SabjaMahfuzur R Sarker
Nov 1, 2011·Journal of Bacteriology·Liming LiuJian Chen
Mar 17, 2012·BMC Microbiology·Irene S LøvdalPer Einar Granum
Nov 30, 2010·Trends in Microbiology·Daniel Paredes-SabjaMahfuzur R Sarker
Sep 28, 2010·Research in Microbiology·Christian A Ross, Ernesto Abel-Santos
Mar 20, 2014·Journal of Applied Microbiology·J M CraneG C Bergstrom
Jun 12, 2014·Journal of Applied Microbiology·O M Alzahrani, Anne Moir
Aug 19, 2014·Research in Microbiology·Valeria Olguín-AranedaDaniel Paredes-Sabja
May 22, 2016·Applied and Environmental Microbiology·Kristina Borch-PedersenMarina Aspholm
Jul 27, 2017·3 Biotech·Nimisha TehriPradip Kumar Sharma
Jul 13, 2017·Annual Review of Microbiology·Peter SetlowYong-Qing Li
May 23, 2019·Proceedings of the National Academy of Sciences of the United States of America·Yunfeng LiBing Hao

Related Concepts

Potassium bromide
GrmA protein, Bacillus megaterium
Bacillus megaterium
Bacterial Proteins
Bromides
Chromosomes, Bacterial
DNA, Bacterial
Genes, Bacterial
Cis-Trans Test
Glucose, (beta-D)-Isomer

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