Role of Cln1 during melanization of Cryptococcus neoformans

Frontiers in Microbiology
Rocío García-RodasOscar Zaragoza

Abstract

Cryptococcus neoformans is an opportunistic fungal pathogen that has several well-described virulence determinants. A polysaccharide capsule and the ability to produce melanin are among the most important. Melanization occurs both in vitro, in the presence of catecholamine and indole compounds, and in vivo during the infection. Despite the importance of melanin production for cryptococcal virulence, the component and mechanisms involved in its synthesis have not been fully elucidated. In this work, we describe the role of a G1/S cyclin (Cln1) in the melanization process. Cln1 has evolved specifically with proteins present only in other basidiomycetes. We found that Cln1 is required for the cell wall stability and production of melanin in C. neoformans. Absence of melanization correlated with a defect in the expression of the LAC1 gene. The relation between cell cycle elements and melanization was confirmed by the effect of drugs that cause cell cycle arrest at a specific phase, such as rapamycin. The cln1 mutant was consistently more susceptible to oxidative damage in a medium that induces melanization. Our results strongly suggest a novel and hitherto unrecognized role for C. neoformans Cln1 in the expression of virulence traits.

References

Nov 1, 1979·Journal of Clinical Microbiology·T A Nurudeen, D G Ahearn
Aug 1, 1995·Infection and Immunity·Y WangA Casadevall
Dec 1, 1994·Infection and Immunity·H S EmeryE S Jacobson
Oct 1, 1994·Applied and Environmental Microbiology·Y Wang, A Casadevall
Nov 1, 1993·Journal of Bacteriology·E S Jacobson, S B Tinnell
Aug 1, 1996·The Journal of Experimental Medicine·S D SalasP R Williamson
Aug 15, 1997·FEMS Microbiology Letters·A L Rosas, A Casadevall
Nov 10, 1998·Fungal Genetics and Biology : FG & B·J A AlspaughJ Heitman
Oct 12, 2000·Clinical Microbiology Reviews·E S Jacobson
Apr 12, 2001·Journal of Applied Microbiology·A CarreiraV Loureiro
Aug 14, 2001·Infection and Immunity·X ZhuP R Williamson
Dec 12, 2001·Molecular Microbiology·T EricksonP R Williamson
May 25, 2002·Pigment Cell Research·Kazumasa Wakamatsu, Shosuke Ito
Apr 5, 2003·Cellular Microbiology·Joshua D Nosanchuk, Arturo Casadevall
Apr 23, 2004·Biological Procedures Online·Oscar Zaragoza, Arturo Casadevall
Aug 5, 2005·Microbiology·Federico Navarro-GarcíaJesús Pla
Aug 17, 2005·Molecular Microbiology·Felicia J WaltonJoseph Heitman
Oct 24, 2006·Current Opinion in Microbiology·Judith Berman
Oct 27, 2007·Nucleic Acids Research·Jaime Huerta-CepasToni Gabaldón
Dec 1, 2007·Microbiology·Helene C EisenmanArturo Casadevall
Sep 13, 2008·Revista iberoamericana de micología·Eidi Alvarado-RamírezValerio Vidotto
Nov 29, 2011·Biochemical and Biophysical Research Communications·Eric V VirtudazoSusumu Kawamoto
Dec 17, 2011·Applied Microbiology and Biotechnology·Helene C Eisenman, Arturo Casadevall
Apr 9, 2013·Revista Do Instituto De Medicina Tropical De São Paulo·Renan Marrichi MauchAmanda Latercia Tranches Dias
Oct 1, 2014·Fungal Genetics and Biology : FG & B·Carolin WollschlaegerGuilhem Janbon

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Citations

Feb 6, 2020·MBio·Radames J B CorderoArturo Casadevall
Nov 10, 2020·Science China. Life Sciences·Pengjie HuLinqi Wang
Apr 4, 2021·Bioorganic & Medicinal Chemistry Letters·Mostafa TeymuriMehdi Razzaghi-Abyaneh

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Methods Mentioned

BETA
Protein Assay
flow cytometry
PCR

Software Mentioned

Graph Pad Prism
CellQuest
PhylomeDB
FlowJo

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