Role of competition for nutrients in suppression of Clostridium difficile by the colonic microflora.

Infection and Immunity
K H Wilson, F Perini

Abstract

The cecal flora of mice is able to eliminate Clostridium difficile from the mouse cecum even when C. difficile is the first organism established. We used a continuous-flow (CF) culture model of the cecal flora to investigate the possibility that competition for nutrients is one mechanism for this antagonism. The medium for the CF cultures consisted of homogenates of fecal pellets from germfree mice. Carbohydrate analysis showed that mouse flora depleted 74 to 99.8% of the various carbohydrates from this environment-simulating medium. When inoculated into filtrates made from CF cultures of mouse flora, C. difficile multiplied slower than the dilution rate of the CF cultures unless glucose, N-acetylglucosamine, or N-acetylneuraminic acid was added. C. difficile did not synthesize hydrolytic enzymes able to cleave these monosaccharides from oligosaccharide side chains. As found previously, veal infusion broth did not support the growth of a microflora that could be transferred to gnotobiotic mice and fully suppress C. difficile. When mucin or monosaccharides found in mucin were added to veal infusion broth, the flora functioned normally in this regard. These data suggest that as yet unidentified organisms compete more efficiently ...Continue Reading

Associated Clinical Trials

Citations

Oct 20, 2001·The Annals of Otology, Rhinology, and Laryngology·R H FreeF W Albers
Apr 15, 2004·The Journal of International Medical Research·F SalviniM Giovannini
Apr 7, 2006·Expert Review of Anti-infective Therapy·Shira Doron, Sherwood L Gorbach
May 19, 2006·Journal of Pediatric Gastroenterology and Nutrition·H SzajewskaStefano Guandalini
May 15, 2007·Applied and Environmental Microbiology·Maira Jessica Medellin-PeñaMansel W Griffiths
Mar 5, 2016·Gut Microbes·Jason M RidlonPhillip B Hylemon
May 1, 1995·Biological Reviews of the Cambridge Philosophical Society·H Smith
Oct 15, 1998·The American Journal of Gastroenterology·S NairC S Pitchumoni
Jan 1, 1997·Scandinavian Journal of Gastroenterology. Supplement·R Fuller, G R Gibson
May 8, 2001·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·M I Alvarez-Olmos, R A Oberhelman
Sep 1, 2005·Alimentary Pharmacology & Therapeutics·H Szajewska, J Mrukowicz
May 5, 2006·Veterinary Pathology·M K Keel, J G Songer
Apr 30, 2005·The Journal of Antimicrobial Chemotherapy·Simon D BainesMark H Wilcox
Nov 5, 2005·Applied and Environmental Microbiology·Sandra MacfarlaneGeorge T Macfarlane
Aug 24, 2013·Pediatric Critical Care Medicine : a Journal of the Society of Critical Care Medicine and the World Federation of Pediatric Intensive and Critical Care Societies·Suresh KumarM Jayashree
Dec 18, 2012·Immunology·Trevor D Lawley, Alan W Walker
May 23, 2015·Frontiers in Cellular and Infection Microbiology·Purnima S Kumar, Matthew R Mason
Oct 22, 2015·Annual Review of Microbiology·C M Theriot, Vincent B Young
Jan 2, 2015·American Journal of Physiology. Gastrointestinal and Liver Physiology·Melinda A EngevikRoger T Worrell
Sep 2, 2015·Infection and Immunity·Ekaterina G SemenyukAdam Driks
Nov 12, 2015·Journal of Medical Microbiology·Christophe Le LayIsmaı L Fliss
Aug 18, 2018·Gut Microbes·James CollinsRobert A Britton
Apr 1, 1991·Gut·R Fuller
Jan 1, 1996·Scandinavian Journal of Infectious Diseases·P O StotzerH Abrahamsson
Sep 18, 1998·Journal of Pediatric Gastroenterology and Nutrition·J A Vanderhoof, R J Young
May 22, 2008·Journal of Pediatric Gastroenterology and Nutrition·Andrew R BarclayLawrence T Weaver
Apr 12, 2014·Cellular Microbiology·Jessica A FerreyraJustin L Sonnenburg
Oct 27, 2015·Nature Reviews. Microbiology·Gregory P DonaldsonSarkis K Mazmanian
Feb 26, 2016·The Journal of Infectious Diseases·Sahil KhannaElizabeth L Hohmann
Jun 23, 2016·Nature Reviews. Gastroenterology & Hepatology·Alexander Khoruts, Michael J Sadowsky

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Hereditary Sensory Autonomic Neuropathy

Hereditary Sensory Autonomic Neuropathies are a group of inherited neurodegenerative disorders characterized clinically by loss of sensation and autonomic dysfunction. Here is the latest research on these neuropathies.

Glut1 Deficiency

Glut1 deficiency, an autosomal dominant, genetic metabolic disorder associated with a deficiency of GLUT1, the protein that transports glucose across the blood brain barrier, is characterized by mental and motor developmental delays and infantile seizures. Follow the latest research on Glut1 deficiency with this feed.

Regulation of Vocal-Motor Plasticity

Dopaminergic projections to the basal ganglia and nucleus accumbens shape the learning and plasticity of motivated behaviors across species including the regulation of vocal-motor plasticity and performance in songbirds. Discover the latest research on the regulation of vocal-motor plasticity here.

Neural Activity: Imaging

Imaging of neural activity in vivo has developed rapidly recently with the advancement of fluorescence microscopy, including new applications using miniaturized microscopes (miniscopes). This feed follows the progress in this growing field.

Nodding Syndrome

Nodding Syndrome is a neurological and epileptiform disorder characterized by psychomotor, mental, and growth retardation. Discover the latest research on Nodding Syndrome here.

LRRK2 & Microtubules

Mutations in the LRRK2 gene are risk-factors for developing Parkinson’s disease (PD). LRRK2 mutations in PD have been shown to enhance its association with microtubules. Here is the latest research.